Project description:Two induction systems, one based on the destablization domain (FKBP) and the other based on mutated ligand-binding domain of the estrogen receptor, require constant application of Shld1 and 4-hydroxytamoxifen (4OHT) to cells respectively, so we sought to quantify the extent to which these chemical inducers affect transcriptional networks. RW4 mouse ES cells were treated with Shld1 and 4-OHT respectively for 2 days and then subjected to gene expression profiling by microarray. Cells mock-treated with 95% ethanol as vehicle were used as controls. For each condition, two biological replicates were performed .
Project description:A transcriptome study in mouse hematopoietic stem cells was performed using a sensitive SAGE method, in an attempt to detect medium and low abundant transcripts expressed in these cells. Among a total of 31,380 unique transcript, 17,326 (55%) known genes were detected, 14,054 (45%) low-copy transcripts that have no matches to currently known genes. 3,899 (23%) were alternatively spliced transcripts of the known genes and 3,754 (22%) represent anti-sense transcripts from known genes.
