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Embryonic exposure to 100 µg/L lead results in sex-specific expression changes in genes associated with neurological system and cancer in the aged adult zebrafish brain

ABSTRACT: Embryonic exposure to 100 µg/L lead results in sex-specific expression changes in genes associated with neurological system and cancer in the aged adult zebrafish brain

PROVIDER: PRJNA314269 | ENA |

REPOSITORIES: ENA

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Embryonic exposure to 100 µg/L lead results in sex-specific expression changes in genes associated with neurological system and cancer in the aged adult zebrafish brain [female]

Project description:Developmental lead (Pb) exposure has been linked to neurological health issues appearing in children. Results from non-human primate studies also suggest detrimental effects of an early life Pb exposure on neurological health, showing a pathological evidence of Alzheimer’s disease in the aged animal brain. To elucidate the impacts of a developmental Pb exposure on neurological health-related molecular changes occurring in the aged brain, aged zebrafish with a control treatment or an embryonic exposure to 100 µg/L Pb were subjected to a zebrafish-specific microarray analysis by sex. Gene ontology analysis showed alterations in distinct sets of genes associated with nervous system development and function in each sex. In aged adult female zebrafish, significant changes in expression profiles occurred in a number of genes involved in neurological disease and nervous system development and function. On the other hand, in aged adult males, a number of genes with significant expression alterations were found to be associated with cancer and tumors.

2017-11-30 | GSE78880 | GEO

Embryonic exposure to 100 µg/L lead results in sex-specific expression changes in genes associated with neurological system and cancer in the aged adult zebrafish brain [male]

2017-11-30 | GSE78879 | GEO

Embryonic exposure to 10 µg/L lead results in female-specific expression changes in genes associated with nervous system development and function and Alzheimer’s disease in aged adult zebrafish brain

Project description:A developmental lead (Pb) exposure has been proposed as an environmental risk factor for adult neurodegenerative diseases including Alzheimer’s disease (AD). Recent animal studies showed pathological characteristics of AD in adults with a developmental Pb exposure, but additional studies are needed to investigate this phenomenon. To further assess the relationship between an embryonic Pb exposure and latent neurological alterations, the brain of adult female and male zebrafish aged 12 months that were exposed to a control treatment or 10 µg/L Pb only during embryogenesis (1- 72 hours after fertilization) were analyzed on a zebrafish-specific microarray platform. Gene ontology and pathway analysis revealed similarities in the top diseases and functional categories in both sexes, but females had 4.3 times more genes altered than males. In addition, alterations in genes associated with nervous system development and function were more pronounced with a set of 89 genes associated with AD including amyloid precursor protein (APP), apolipoprotein (APOE), and sortlin-related receptor precursor (SORL1) observed to be changed in adult females. Our observations suggest that an embryonic exposure to Pb at levels as low as 10 μg/L disturb global gene expression patterns in a sex-specific manner that could lead to neurological alterations in later life. With these findings, future studies investigating the adverse neurological outcomes of these changes in gene expression will facilitate our understanding of the impact of an embryonic 10 μg/L Pb exposure on neurological disease pathogenesis and the inclusion of additional concentrations will broaden our knowledge of dose-dependent changes.

2016-02-08 | GSE74037 | GEO

Embryonic exposure to 10 µg/L lead results in male-specific expression changes in genes associated with nervous system development and function and Alzheimer’s disease in aged adult zebrafish brain

2016-02-08 | GSE74038 | GEO

Acinetobacter oleivorans DR1 : Cell treated Ampicillin 100 µg/ml in nutrient media

Project description:Transcriptional profiling of A. oleivorans DR1 treated Ampicillin 100 µg/ml for 15min .

2014-06-03 | GSE58166 | GEO

Embryonic exposure to 10 µg/L lead results in sex-specific expression changes in aged adult zebrafish brain

Project description:Embryonic exposure to 10 µg/L lead results in sex-specific expression changes in aged adult zebrafish brain

| PRJNA298829 | ENA

Embryonic exposure to 100 µg/L lead results in sex-specific expression changes in genes associated with neurological system and cancer in the aged adult zebrafish brain

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-11-30 | GSE78881 | GEO

Global Gene Expression Analysis Reveals Dynamic and Developmental Stage–Dependent Enrichment of Lead-Induced Neurological Gene Alterations

Project description:Background: The underlying genetic mechanisms specific to subtle neurological alterations associated with environmental lead (Pb) exposures have not been clearly elucidated. Objectives: The goal of this study was to identify novel gene targets and the underlying genetic mechanisms associated with developmental Pb neurotoxicity. Methods: We first exposed zebrafish embryos to a range of Pb concentrations throughout early development to establish relative toxicity. Using the data from that experiment, we exposed another group of zebrafish embryos to a sublethal dose of Pb (100 ppb) immediately after fertilization through 72 or 120 hr postfertilization (hpf). Global gene expression was then analyzed for molecular pathways and gene ontology enrichment, and Western blot analysis was performed to investigate the translation of gene expression changes to protein levels. Results: After 72 hpf, we identified 231 probes representing 90 nonredundant genes with well-established function or orthology to human genes as being altered by Pb exposure. This gene set was both confirmatory and novel in nature and was highly enriched for neurological development, function, and disease. Moreover, gene changes at this time point were correlated to altered protein levels. Alternatively, the gene set at 120 hpf did not share association with neurological development. Conclusions: Global gene expression alterations associated with developmental Pb exposure were dynamic and dependent on developmental stage. Gene expression alterations at the 72‑hpf time point were highly enriched with genes and molecular pathways associated with neurological development and disease. Moreover, we identified a number of novel targets for future exploration

2018-07-20 | GSE117399 | GEO

Embryonic exposure to 10 Âµg/L lead results in female-specific expression changes in genes associated with nervous system development and function and Alzheimerâs disease in aged adult zebrafish brain

Project description:A developmental lead (Pb) exposure has been proposed as an environmental risk factor for adult neurodegenerative diseases including Alzheimerâs disease (AD). Recent animal studies showed pathological characteristics of AD in adults with a developmental Pb exposure, but additional studies are needed to investigate this phenomenon. To further assess the relationship between an embryonic Pb exposure and latent neurological alterations, the brain of adult female and male zebrafish aged 12 months that were exposed to a control treatment or 10 Âµg/L Pb only during embryogenesis (1- 72 hours after fertilization) were analyzed on a zebrafish-specific microarray platform. Gene ontology and pathway analysis revealed similarities in the top diseases and functional categories in both sexes, but females had 4.3 times more genes altered than males. In addition, alterations in genes associated with nervous system development and function were more pronounced with a set of 89 genes associated with AD including amyloid precursor protein (APP), apolipoprotein (APOE), and sortlin-related receptor precursor (SORL1) observed to be changed in adult females. Our observations suggest that an embryonic exposure to Pb at levels as low as 10 Î¼g/L disturb global gene expression patterns in a sex-specific manner that could lead to neurological alterations in later life. With these findings, future studies investigating the adverse neurological outcomes of these changes in gene expression will facilitate our understanding of the impact of an embryonic 10 Î¼g/L Pb exposure on neurological disease pathogenesis and the inclusion of additional concentrations will broaden our knowledge of dose-dependent changes. A developmental exposure to 10ppb Pb induced global gene expression changes were analyzed in 12 months aged male and female zebarfish brains. A developmental exposure was done shortly after fertilization through 72 hours after fertilization.

2016-02-08 | E-GEOD-74037 | biostudies-arrayexpress

Embryonic exposure to 10 Âµg/L lead results in male-specific expression changes in genes associated with nervous system development and function and Alzheimerâs disease in aged adult zebrafish brain

2016-02-08 | E-GEOD-74038 | biostudies-arrayexpress