Project description:Comparative transcriptomic analysis of Arabidopsis thaliana lines expressing a constitutively active YDA protein (CA-YDA; in La-0 background), and wild-type plants (La-0) non-infected or infected with the necrotrophic fungal pathogen Plectosphaerella cucumerina BMM (PcBMM)
Project description:Comparative transcriptomic analysis of Arabidopsis thaliana yda11 plants (in Col-0 background), and wild-type plants (Col-0) non-infected or infected with the necrotrophic fungal pathogen Plectosphaerella cucumerina BMM (PcBMM)
Project description:Comparative transcriptional profiling of Arabidopsis yda11 mutant and wild-type plants after infection with Plectosphaerella cucumerina
Project description:Comparative transcriptional profiling of Arabidopsis arr6-3 mutant and wild-type (Col-0) plants mock-treated and after infection with Plectosphaerella cucumerina BMM
Project description:To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina.
Project description:Cytokinin signaling pathway, that is mediated by Arabidopsis Response Regulators (ARRs) proteins, has gained functional relevance in the modulation of plant disease resistance responses. We describe here a novel function of ARR6 in the regulation of Arabidopsis immunity and cell wall composition. arr6 mutants were found to be more susceptible and resistant, respectively, to the vascular bacteria Ralstonia solanacearum GMI1000 and the necrotrophic fungus Plectosphaerella cucumerina BMM. In contrast, transgenic Arabidopsis lines overexpressing ARR6 showed the opposite phenotypes, further supporting a role of ARR6 in regulating disease resistance responses. Transcriptomics and metabolomics analyses of arr6 revealed that canonical immune pathways, like those modulated by defensive phytohormones or triggered by Microbe-Associated Molecular Patterns, are not altered in arr6 plants, suggesting that novel, uncharacterized defensive pathways explain arr6-mediated resistance. We identified alterations in the composition of arr6 cell walls in comparison to that of wild-type plants walls. Remarkably, pectin-enriched cell wall fractions extracted from arr6 walls trigger more intense immune responses than those activated by the corresponding wild-type fractions. Our data suggest that arr6 pectin wall fraction contained specific pentose-based oligosaccharides that would function as non-yet described wall-related Damage-Associated Molecular Patterns (DAMPs). These data supported a novel function of ARR6 in the regulation of cell-wall mediated immunity and reinforce the relevance of plant cell wall composition in the modulation of specific disease resistance responses.