Project description:The tick midgut is the main tissue involved in blood feeding and the first organ to have contact with pathogens ingested through the blood meal. We characterized the early transcriptional changes in the midgut of O. moubata in the unfed, engorged and Borrelia duttonii-infected state. The aim is to identify transcripts that are differentially expressed in the respective physiological state.
Project description:To understand differences of gene expression profiles between Francisella strains RNA profiles of Francisella strains were generated by deep sequencing, in triplicate, using NovaSeq6000. qRT–PCR validation was performed using SYBR Green assays. Our study represents the first detailed differential transcriptomic analysis of Francisella strains , with biologic replicates, generated by RNA-seq technology.