Project description:To elucidate a mechanism for drought adaptation of Nostoc flagelliforme, we performed a global phosphoproteome analysis on the abundance of phosphoproteinse. A total of 329 phosphopeptides from 271 phosphoproteins with 1168 phosphorylation sites were identified. The identified DEPPs were functionally categorized to be mainly involved in a two-component signaling pathway, photosynthesis, energy and carbohydrate metabolism, and an antioxidant system.
Project description:Nostoc flagelliforme is able to recover biological activities within hours from months of anhydrobiosis state. In this work, we first constructed a smORF database, and analyzed the proteome of N. flagelliforme over rehydration.
Project description:Change in gene expression for a wild-type (Nostoc punctiforme ATCC 29133) and hmpD-deletion strain (UCD 543) of Nostoc punctiforme ATCC 29133 over the time course of hormogonium development This study is further descirbed in Risser, D.D. and Meeks, J.C. 2013. Comparative transcriptomics with a motility deficient mutant leads to identification of a novel polysaccharide secretion system in Nostoc punctiforme. Molecular Microbiology
Project description:Change in gene expression for a wild-type (Nostoc punctiforme ATCC 29133) and hmpD-deletion strain (UCD 543) of Nostoc punctiforme ATCC 29133 over the time course of hormogonium development This study is further descirbed in Risser, D.D. and Meeks, J.C. 2013. Comparative transcriptomics with a motility deficient mutant leads to identification of a novel polysaccharide secretion system in Nostoc punctiforme. Molecular Microbiology Total RNA from 3 biological replicates at each time point from 0 to 24 hours after hormogonium induction was converted to cDNA, dye-labled and hybridized to nimblegen 12x135k array slides
