Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Staphylococcus aureus

ABSTRACT: Mode of action analysis: Distinct Staphylococcus aureus transcriptome changes upon antivirulence lactone treatment

PROVIDER: PRJNA315768 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR3262520.fastq.gz	Fastqsanger.gz
	SRR3262521.fastq.gz	Fastqsanger.gz
	SRR3262522.fastq.gz	Fastqsanger.gz
	SRR3262523.fastq.gz	Fastqsanger.gz
	SRR3262524.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Staphylococcus aureus

Project description:Mode of action analysis: Distinct Staphylococcus aureus transcriptome changes upon antivirulence hydroxy amide treatment

| PRJNA271787 | ENA

Transcriptional responses of CDCA treated Staphylococcus aureus cells

Project description:Analysis of Staphylococcus aureus treated by CDCA. Staphylococcus aureus cells are evaluated with RNA-seq to understand the genes affected by this antibacterial agent. Our results provide new vision on the mode of action by CDCA.

2022-10-15 | GSE193011 | GEO

Transcriptional responses of Tanreqing treated Staphylococcus aureus cells

Project description:Analysis of Staphylococcus aureus treated by Tanreqing. Staphylococcus aureus cells are evaluated with RNA-seq to understand the genes affected by this antibacterial agent. Our results provide new vision on the mode of action by Tanreqing.

2021-06-07 | GSE162107 | GEO

A quantitative map of beta-lactone induced virulence regulation

Project description:Beta-lactones have recently been introduced as the first selective ClpP inhibitors that attenuate virulence of both sensitive Staphylococcus aureus and multiresistant strains (MRSA). Although previous knockout studies showed that ClpP is essential for S. aureus alpha-toxin production a link between beta-lactone inhibition and molecular virulence mechanisms has been lacking so far. We here perform a chemical-proteomic approach to elucidate anti-virulence pathways. First, we demonstrate by gel-free activity-based protein profiling that ClpP is the predominant target of beta-lactones. Only a few off-targets were discovered, which, unlike ClpP, were not involved in the reduction of alpha-toxin expression. Second, in-depth mechanistic insight was provided by a full proteomic comparison between lactone treated and untreated S. aureus cells. Quantitative mass spectrometric analysis revealed a strong up-regulation of Rot and a corresponding down-regulation of alpha-toxin, providing the first direct connection between the lactone-dependent phenotype and a corresponding cellular mechanism. By building up a quantitative virulence regulation network, we visualize the impact of ClpP inhibition in a systems biology context. Interestingly, a lack of in vitro Rot degradation by either ClpXP or ClpCP calls for an indirect mode of action that may involve ClpX-mediated RNA signaling and feed-back circuits.

2017-02-08 | PXD004680 | Pride

Extracellular biofilm matrix proteins in Staphylococcus aureus

Project description:Comparison of biofilm versus planktonic mode of growth in Staphylococcus aureus

2014-01-18 | E-MEXP-3924 | biostudies-arrayexpress

Antimicrobial activity of graphene oxide is associated with alteration in stress response and membrane proteins

Project description:The aim of this study was to explore the mode of action of GO, via an in-depth proteomics analysis, on the protein expression of targeted bacteria. For this purpose, samples of Staphylococcus aureus were grown in the presence of GO and samples were collected from different growth phases to examine cell viability and to analyze the changes in protein expression.

2023-01-19 | PXD036796 | Pride

Global transcriptional profiling of Staphylococcus aureus treated with rhein

Project description:Staphylococcus aureus is one of the most important pathogens in humans and animals, multiply resistant strains are increasingly widespread, new agents are needed for the treatment of S. aureus. Rhein, a natural plant product, has potential antimicrobial activity against Staphylococcus aureus. We employed Affymetrix Staphylococcus aureus GeneChipsTM arrays to investigate the global transcriptional profiling of Staphylococcus aureus ATCC25923 treated with rhein. Results provided insight into mechanisms involved in rhein - Staphylococcus aureus interactions. Keywords: rhein response

2007-06-15 | GSE8135 | GEO

Mode of action analysis: Distinct Staphylococcus aureus transcriptome changes upon antivirulence lactone treatment

Project description:To elucidate the antivirulent lactone U1 mode of action, next generation sequencing was applied to analyze the transcriptome of NCTC 8325 cells treated with either compound or DMSO as control.

2018-01-01 | GSE79407 | GEO

Global transcriptional profiling of Staphylococcus aureus treated with magnolol

Project description:Staphylococcus aureus (S. aureus) is an important human and animal pathogen, multiply resistant strains are increasingly widespread, new agents are needed for the treatment of S. aureus. magnolol has potent antimicrobial activity against S. aureus. We employed Affymetrix Staphylococcus aureus GeneChipsTM arrays to investigate the global transcriptional profiling of Staphylococcus aureus ATCC25923 treated with magnolol. Keywords: gene expression array-based, count

2008-10-22 | GSE13236 | GEO

Global transcriptional profiling of Staphylococcus aureus treated with sodium houttuyfonate

Project description:Staphylococcus aureus (S. aureus) is an important human and animal pathogen, multiply resistant strains are increasingly widespread, new agents are needed for the treatment of S. aureus. sodium houttuyfonate has potent antimicrobial activity against S. aureus. We employed Affymetrix Staphylococcus aureus GeneChipsTM arrays to investigate the global transcriptional profiling of Staphylococcus aureus ATCC25923 treated with sodium houttuyfonate. Keywords: gene expression array-based, count

2008-10-22 | GSE13233 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data