Project description:Effect of thalamic deletion of mouse Sox2, or Nr2f1, on gene expression in the visual thalamus (dorsolateral geniculate nucleus, dLGN), as described in "SOX2 and NR2F1 coordinate the gene expression program of the early postnatal visual thalamus" (bioRxiv doi number to be updated) To address the effect of Sox2, or Nr2f1, Cre-mediated thalamic deletion in mouse, on gene expression in the visual thalamus (dLGN), we dissected the dLGN from mutant (Sox2, or Nr2f1) and control mice at postnatal day 0 (P0), for both Sox2 and Nr2f1 mutants. We then performed gene expression analysis by RNAseq.
Project description:CUT&RUN was performed for Sox2 on ex-vivo dissected visual thalamic nuclei from P0 mice, revealing context specific activity of Sox2 binding in differentiated neurons.
Project description:We performed transcriptome profiling for mouse dLGN (dorsolateral geniculate nucleus) tissues derived from P3, P7, P14 and P23 WT and Math5-/- mice.
