Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A multidimensional blood stimulation assay reveals immune alterations underlying systemic juvenile idiopathic arthritis

ABSTRACT: A multidimensional blood stimulation assay reveals immune alterations underlying systemic juvenile idiopathic arthritis

PROVIDER: PRJNA318587 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6006880_1.fastq.gz	Fastqsanger.gz
	SRR6006880_2.fastq.gz	Fastqsanger.gz
	SRR6006890_1.fastq.gz	Fastqsanger.gz
	SRR6006890_2.fastq.gz	Fastqsanger.gz
	SRR6006900_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 64

Similar Datasets

A multidimensional blood stimulation assay reveals immune alterations underlying systemic juvenile idiopathic arthritis [RNA-Seq]

Project description:Despite great scientific and technological advances, immune alterations predisposing to sporadic human inflammatory diseases remain mostly unknown. To fill this gap, we developed a strategy to evaluate blood leukocyte responses to innate stimuli, simultaneously at the transcriptional, cellular and secreted protein levels. The data were integrated using weighted gene co-expression network analysis. When applied to systemic juvenile idiopathic arthritis (sJIA), an autoinflammatory disease of unknown etiology, this approach identified gene sets associated with specific cytokine environments and activated leukocyte subsets. During the remission phase of disease and off treatment, sJIA patients displayed dysregulated responses to TLR4, TLR8 and TLR7 stimulation in blood. Isolated sJIA monocytes accumulated higher levels of intracellular IL-1β after stimulation, and underexpressed the IL-1 inhibitor AHR at baseline. In line with the recent demonstration that AHR downregulation in monocytes is linked to macrophage differentiation, we show that the differentiation of sJIA monocytes in vitro was skewed towards macrophages, away from dendritic cell phenotype. This might contribute to the increased incidence of macrophage activation syndrome in these patients. The integrated analysis of these high-dimensional data can thus help unravel underlying immune alterations predisposing to complex inflammatory diseases.

2017-09-19 | GSE103501 | GEO

Remission in Polyarticular Juvenile Idiopathic Arthritis

Project description:Identify biomarkers to predict response to therapy in polyarticular juvenile idiopathic arthritis (JIA) using gene expression microarrays.

2009-04-13 | GSE15645 | GEO

Molecular profiles of Systemic Juvenile Idiopathic Arthritis patients

Project description:Biomarker identification for diagnosis of systemic juvenile idiopathic arthritis (SJIA), an auto-inflammatory disease that presents with prolonged fevers.

2015-09-03 | GSE57183 | GEO

A multidimensional blood stimulation assay reveals immune alterations underlying systemic juvenile idiopathic arthritis

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-09-18 | GSE80325 | GEO

Remission in Polyarticular Juvenile Idiopathic Arthritis

Project description:Identify biomarkers to predict response to therapy in polyarticular juvenile idiopathic arthritis (JIA) using gene expression microarrays. 42 samples from 13 controls, 14 active patients, 9 patients in clinical remission with medication (CRM), and 6 patients in clinical remission without medication (CR). All patients had polyarticular JIA.

2009-04-13 | E-GEOD-15645 | biostudies-arrayexpress

A multidimensional blood stimulation assay reveals immune alterations underlying systemic juvenile idiopathic arthritis.

Project description:The etiology of sporadic human chronic inflammatory diseases remains mostly unknown. To fill this gap, we developed a strategy that simultaneously integrates blood leukocyte responses to innate stimuli at the transcriptional, cellular, and secreted protein levels. When applied to systemic juvenile idiopathic arthritis (sJIA), an autoinflammatory disease of unknown etiology, this approach identified gene sets associated with specific cytokine environments and activated leukocyte subsets. During disease remission and off treatment, sJIA patients displayed dysregulated responses to TLR4, TLR8, and TLR7 stimulation. Isolated sJIA monocytes underexpressed the IL-1 inhibitor aryl hydrocarbon receptor (AHR) at baseline and accumulated higher levels of intracellular IL-1β after stimulation. Supporting the demonstration that AHR down-regulation skews monocytes toward macrophage differentiation, sJIA monocytes differentiated in vitro toward macrophages, away from the dendritic cell phenotype. This might contribute to the increased incidence of macrophage activation syndrome in these patients. Integrated analysis of high-dimensional data can thus unravel immune alterations predisposing to complex inflammatory diseases.

| S-EPMC5679164 | biostudies-literature

Molecular profiles of Systemic Juvenile Idiopathic Arthritis patients

Project description:Biomarker identification for diagnosis of systemic juvenile idiopathic arthritis (SJIA), an auto-inflammatory disease that presents with prolonged fevers. Disease vs. healthy control

2015-09-03 | E-GEOD-57183 | biostudies-arrayexpress

Neutrophil gene expression in systemic juvenile idiopathic arthritis

Project description:Neutrophil gene expression in systemic juvenile idiopathic arthritis

| PRJNA505535 | ENA

Juvenile idiopathic arthritis: peripheral blood cells

Project description:Systemic-onset juvenile idiopathic arthritis (soJIA) is a rheumatic disease in childhood characterized by systemic symptoms and a relatively poor prognosis. The peripheral leukocytes are thought to play the pathological role of soJIA although the exact cause is still obscure. In this study, we aimed to clarify the cellular functional abnormality in those cells. Here, we analyzed the gene expression profile in peripheral leukocytes from 51 patients with soJIA, 6 patients with poly-articular type JIA (polyJIA) and 8 healthy children utilizing DNA microarrays. A total of 3,491 genes, including genes related to immune reponse and metabolism, were differentially expressed in patients with soJIA compared to healthy individuals. The result provides insight into the pathogenesis of soJIA. Keywords: disease state analysis

2008-07-01 | GSE8361 | GEO

Juvenile idiopathic arthritis: peripheral blood cells

2010-05-26 | E-GEOD-8361 | biostudies-arrayexpress