Project description:Gene expression of a canopy-forming kelp, Eisenia bicyclis (Laminariales, Phaeophyceae), under high temperature stress
| PRJDB12239 | ENA
Project description:Phaeoviruses present in cultured and natural kelp species, Saccharina latissima and Laminaria hyperborea (Phaeophyceae, Laminariales), in Norway
Project description:The data is a result of a large laboratory experiment targeting interactive effects of salinity and temperature on the transcriptomic level in algae from two populations of an ecologically relevant kelp species (Laminariales), Saccharina latissima. Research interest in S. latissima has recently been increasing given its importance as ecosystem engineer along temperate rocky shores in the Atlantic Ocean, and its growing potential in industrial applications such as aquaculture, pharmaceutics, food and feed. Young sporophytes of S. latissima were raised from stock cultures of clonal male and female gametophytes at the Alfred-Wegener-Institute Helmholtz Centre for Polar and Marine Research. Cultures originated from sporophyte collected at Kongsfjorden (79°N, 11°E; Spitsbergen, Norway) and at Roscoff (48° 43′ 39″ N, 3° 59′ 13.2″ W; Brittany, France). Sporophytes of both populations were grown aerated in glass beakers at 8°C and under a photon fluence rate of 20 µmol photons m–2 s–1 of photosynthetically active radiation with a 18 h light: 6 h dark photoperiod and were cultivated in sterile seawater enriched with Provasoli (Starr & Zeikus 1993) with an absolute salinity (SA) of ~30 during three months. At the start of the experiment, sporophytes were either kept at 8°C (or transferred to 0°C and 15°C) in temperature controlled rooms. After one week, per each temperature, sporophytes were divided into a low salinity treatment of SA 20 or kept at the control salinity (SA 30).
Project description:We produced an extensive transcript catalog for LCLs of 5 primate species by leveraging isoform sequencing and short-read RNA-seq. The curated transcriptomes were used to assist mass spectrometry protein identifications.
Project description:This study describes the combined sequencing of the genomes and transcriptomes of single blastomeres from mouse 8-cell stage embryos.
Project description:Here, we systematically profiled clinical phenomes, transcriptomes, proteomes, metabolomes, and immune repertoire of blood cells or plasma in over 1000 specimens from SARS-CoV-2 Omicron patients across multiple disease phases.
Project description:Advances in platelet transcriptomics allow prediction of the full human platelet proteome. We integrated proteomes from six cohorts, encompassing 5.2k unique proteins (3.5k with copy numbers), with novel genome-wide platelet and megakaryocyte transcriptomes (Blueprint).