Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RsrR: a novel redox sensitive Rrf2 family transcription factor in Streptomyces venezuelae

ABSTRACT: RsrR: a novel redox sensitive Rrf2 family transcription factor in Streptomyces venezuelae

PROVIDER: PRJNA320530 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Streptomyces venezuelae ATCC 10712

Project description:Streptomyces venezuelae RsrR ChIP-seq

| PRJNA315371 | ENA

RsrR: a novel redox sensitive Rrf2 family transcription factor in Streptomyces venezuelae

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-02-12 | GSE81105 | GEO

ChIP-Seq data of the Streptomyces venezualae protein Sven6563 (RsrR)

Project description:We report the results of ChIP-Seq experiment investigating the Rrf2 family transciptional regulator RsrR (putatively name Redox sensitive response regulator). The experiment utilized an in trans copy of rsrR with an N-terminal 3xFlag tag encoded on the plasmid pMS82 (containing a phiBT1 integration site). The experiment utilized beads associated with anti-flag antibodies, specific for the Flag tagged protein and a WT host strain. We report >600 target binding sites for the RsrR regulon encompassing a broad range of functional targets with specific reference to those associate with NADPH and NADH metabolism and synthesis.

2017-02-12 | GSE81073 | GEO

ChIP-exo data of the Streptomyces venezualae protein Sven6563 (RsrR)

Project description:We report the results of ChIP-Seq experiment investigating the Rrf2 family transciptional regulator RsrR (putativeily name Redox sensitive response regulator). The experiment utilised an in trans copy of rsrR with an N-terminal 3xFlag tag encoded on the plasmid pMS82 (containing a phiBT1 integration site). The experiment utilised beads associated with anti-flag antibodies, specific for the Flag tagged protein and a WT host strain. Exo nuclease treatment was carried prior to immunoprecipitation to improve the resolution of the binding site. We report >600 target binding sites for the RsrR regulon encompasing a broad range of functional targets with specific refernce to those associate with NADPH and NADH metabolism and synthesis.

2017-02-12 | GSE80818 | GEO

Identifying Lsr2 binding sites in Streptomyces venezuelae

Project description:The goal of this work was to determine where Lsr2 binds within the S. venezuelae chromosome, and to differentiate between direct versus indirect effects by comparing our ChIP-seq results with RNA-seq results

2018-06-09 | GSE115538 | GEO

Redox-sensitive bZIP68 plays a role in balancing stress tolerance with growth in Arabidopsis

Project description:Perturbation of the cellular redox state by stress conditions is sensed by redox-sensitive proteins so that the cell can physiologically respond to stressors; however, the mechanisms linking sensing to response remain poorly understood in plants. Here we report that the transcription factor bZIP68 underwent in vivo oxidation in Arabidopsis cells under oxidative stress which is dependent on its redox-sensitive Cys320 residue. bZIP68 is primarily localized to the nucleus under normal conditions in Arabidopsis seedlings and oxidative stress reduces its accumulation in the nucleus and increases its cytosolic localization. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) revealed that bZIP68 primarily binds to promoter regions containing the core G-box (CACGTG) or G-box-like motif of the genes involved in abiotic and biotic stress responses, photosynthesis, biosynthetic processes, and transcriptional regulation. The bzip68 mutant displayed slower growth but enhanced tolerance to oxidative stress. The results from the ChIP-seq and phenotypic and transcriptome comparison between the bzip68 mutant and wildtype indicate that bZIP68 normally suppresses expression of stress tolerance genes and promotes expression of growth-related genes, whereas its inactivation enhances stress tolerance but suppresses growth. bZIP68 might balance stress tolerance with growth through the extent of its oxidative inactivation according to the environment.

2020-07-01 | GSE133900 | GEO

Streptomyces venezuelae

Project description:Streptomyces venezuelae plasmid-cured strains

| PRJNA638164 | ENA

Streptomyces venezuelae

Project description:ChIP-exo data of the Streptomyces venezualae protein Sven6563 (RsrR)

| PRJNA320079 | ENA

Gene expression analysis of phage Alderaan infected mycelium from S. venezuelae

Project description:To gain insights into the processes underlying adaptation to phage infection, we performed RNA-seq analysis of infected mycelium from the plaque interface of S. venezuelae wild type harvested 24 h and 72 h after infection with Alderaan.

2022-09-22 | GSE213718 | GEO

Streptomyces venezuelae ATCC15439

Project description:Streptomyces venezuelae ATCC15439 RNA-seq

| PRJEB34219 | ENA