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Streptomyces venezuelae ATCC 10712

ABSTRACT: dRNA-seq data of the Streptomyces venezualae WT and mutant (sven6563::apr (rsrR::apr)) strains at time point 16 h

PROVIDER: PRJNA320533 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR3475810.fastq.gz	Fastqsanger.gz
	SRR3475811.fastq.gz	Fastqsanger.gz
	SRR3475808.fastq.gz	Fastqsanger.gz
	SRR3475809.fastq.gz	Fastqsanger.gz

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Streptomyces venezuelae ATCC 10712

Project description:ChIP-Seq data of the Streptomyces venezualae protein Sven6563 (RsrR)

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dRNA-seq data of the Streptomyces venezualae WT and mutant (sven6563::apr (rsrR::apr)) strains at time point 16 h

Project description:We report here the sequence results of S. venezualae WT and the mutant sven6563::apr (rsrR::apr) strains at 16h and the differential expression profile of WT vs. Mutant. Additionally we report the resulting TSSAR analysis of the transcriptional start site information for the genes expressed at this time point.

2017-02-12 | GSE81104 | GEO

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Streptomyces venezuelae ATCC 10712

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New insights into chloramphenicol biosynthesis in Streptomyces venezuelae ATCC 10712.

Project description:Comparative genome analysis revealed seven uncharacterized genes, sven0909 to sven0915, adjacent to the previously identified chloramphenicol biosynthetic gene cluster (sven0916-sven0928) of Streptomyces venezuelae strain ATCC 10712 that was absent in a closely related Streptomyces strain that does not produce chloramphenicol. Transcriptional analysis suggested that three of these genes might be involved in chloramphenicol production, a prediction confirmed by the construction of deletion mutants. These three genes encode a cluster-associated transcriptional activator (Sven0913), a phosphopantetheinyl transferase (Sven0914), and a Na(+)/H(+) antiporter (Sven0915). Bioinformatic analysis also revealed the presence of a previously undetected gene, sven0925, embedded within the chloramphenicol biosynthetic gene cluster that appears to encode an acyl carrier protein, bringing the number of new genes likely to be involved in chloramphenicol production to four. Microarray experiments and synteny comparisons also suggest that sven0929 is part of the biosynthetic gene cluster. This has allowed us to propose an updated and revised version of the chloramphenicol biosynthetic pathway.

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Streptomyces venezuelae ATCC 10712

Project description:Streptomyces venezuelae ATCC 10712 genome sequencing project

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Project description:Streptomyces exploration is triggered by fungal interactions and volatile signals

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RNA-seq data of the Streptomyces venezualae WT and mtrB::apr mutant strains at time point 14 and 20 h (biological duplicates).

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