Project description:We discovered lncRNA PSTAR as a novel hepatocellular carcinoma (HCC) - associated lncRNA, which could interact with hnRNP K protein. To explore the function of PSTAR in HCC and underlying molecular mechanism, we examined the global gene expression change in HepG2 cells with PSTAR knockdown. The gene expression profile of HepG2 with hnRNP K knockdown was also analyzed to investigate the correlationship between PSTAR and hnRNP K. Further, doxorubicin (DOX) was applied to induce DNA damage, and the effect of PSTAR and hnRNP K under DNA damage was also investigated.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
