Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bacteroides ovatus strain:NLAE-zl-C500

ABSTRACT: Bacteroides ovatus NLAE-zl-C500 genome sequencing

PROVIDER: PRJNA323064 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR4228496_1.fastq.gz	Fastqsanger.gz
	SRR4228496_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Bacteroides ovatus strain:NLAE-zl-C57

Project description:Bacteroides ovatus NLAE-zl-C57 genome sequencing

| PRJNA323065 | ENA

Bacteroides thetaiotaomicron strain:NLAE-zl-C579

Project description:Bacteroides thetaiotaomicron NLAE-zl-C579 genome sequencing

| PRJNA323066 | ENA

Bacteroides xylanisolvens strain:NLAE-zl-C202

Project description:Bacteroides xylanisolvens NLAE-zl-C202 genome sequencing

| PRJNA323067 | ENA

Bacteroides xylanisolvens strain:nlae-zl-g339

Project description:Bacteroides xylanisolvens NLAE-zl-G339 genome sequencing

| PRJNA303302 | ENA

Phocaeicola vulgatus strain:nlae-zl-g202

Project description:Bacteroides vulgatus NLAE-zl-G202 genome sequencing

| PRJNA303303 | ENA

Depolymerization of plant cell wall glycans by symbiotic human gut bacteria (Bacteroides ovatus)

Project description:Symbiotic bacteria inhabiting the distal human gut have evolved under intense pressure to utilize complex carbohydrates, predominantly plant cell wall glycans abundant in our diets. These substrates are recalcitrant to depolymerization by digestive enzymes encoded in the human genome, but are efficiently targeted by some of the ~103-104 bacterial species that inhabit this niche. These species augment our comparatively narrow carbohydrate digestive capacity by unlocking otherwise unusable sugars and fermenting them into host-absorbable forms, such as short-chain fatty acids. We used phenotype profiling, whole-genome transcriptional analysis and molecular genetic approaches to investigate complex glycan utilization by two fully sequenced and closely related human gut symbionts: Bacteroides thetaiotaomicron and Bacteroides ovatus. Together these species target all of the common glycosidic linkages found in the plant cell wall, as well as host polysaccharides, but each species exhibits a unique ‘glycan niche’: in vitro B. thetaiotaomicron targets plant cell wall pectins in addition to linkages contained in host N- and O-glycans; B. ovatus uniquely targets hemicellulosic polysaccharides along with several pectins, but is deficient in host glycan utilization. Bacteroides ovatus bacteria were grown either in vitro on defined complex glycan sources, or in vivo in the intestinal tract of gnotobiotic mice fed variable diets. Increased in vitro gene expression was used to indicate the genes required for metabolism of complex glycans and compared to in vivo transcriptional activity to determine expression in the mouse gut.

2011-05-01 | E-GEOD-25575 | biostudies-arrayexpress

RNA-seq of human gut Bacteroides xylanisolvens and Bacteroides ovatus isolates grown on mucin O-linked glycans compared to glucose

Project description:Purpose: Examining the transcriptome of human gut bacteria (Bacteroides xylanisolvens/Bacteroides ovatus) that grow on mucin O-linked glycans as a sole carbon source Methods: Strains were grown on 10 mg/ml mucin O-linked glycans (MOG) or 5 mg/ml glucose as a sole carbon source in vitro. Fold change was calculated as MOG over glucose. Once cells reached an optical density corresponding to mid-log phase growth, RNA was isolated and rRNA depleted. Samples were multiplexed for sequencing on the Illumina HiSeq platform at the University of Michigan Sequencing Core. Data was analyzed using Arraystar software (DNASTAR, Inc.) Genes with significant up- or down-regulation were determined by the following criteria: genes with an average fold-change >10-fold and biological replicates with a normalized expression level >1% of the overall average RPKM expression level. Results: We identified genes activated in response to mucin O-linked glycans from Bacteroides xylanisolvens/Bacteroides ovatus strains

2020-12-01 | GSE155869 | GEO

[Clostridium] innocuum strain:NLAE-zl-C381

Project description:Clostridium innocuum NLAE-zl-C381 genome sequencing

| PRJNA323324 | ENA

Enterocloster clostridioformis strain:NLAE-zl-C196

Project description:Lachnoclostridium clostridioforme NLAE-zl-C196 genome sequencing

| PRJNA322952 | ENA

Faecalicatena contorta strain:NLAE-zl-C134

Project description:Lachnoclostridium contortum NLAE-zl-C134 genome sequencing

| PRJNA322954 | ENA