Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA323743 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptional profiling of mouse comparing in vitro-derived DC progenitors from control and Gata2 conditional knockout mice. Two-condition experiment, Control DCs vs. G2 Knockout DCs. Biological replicates: 4 control, 3 Gata2 knockout, independently grown and harvested. One replicate per array. Dendritic cells (DCs) are critical immune response regulators; however, the mechanism of DC differentiation is not fully understood. Heterozygous germline GATA2 mutations induce GATA2 deficiency syndrome, characterized by monocytopenia, a predisposition to myelodysplasia/acute myeloid leukemia, and a profoundly reduced DC population, which is associated with increased susceptibility to viral infections, impaired phagocytosis, and decreased cytokine production. To define the role of GATA2 in DC differentiation and function, we studied Gata2 conditional knockout and haploinsufficient mice. Gata2 conditional deficiency significantly reduced the DC count, whereas Gata2 haploinsufficiency did not affect this population. GATA2 was required for the in vitro generation of DCs from Linâ??Sca-1+Kit+ cells, common myeloid-restricted progenitors, and common dendritic cell precursors, but not common lymphoid-restricted progenitors or granulocyte-macrophage progenitors, suggesting that GATA2 functions in the myeloid pathway of DC differentiation. Moreover, expression profiling demonstrated reduced expression of myeloid-related genes, including mafb, and increased expression of T-lymphocyte-related genes, including Gata3 and Tcf7, in Gata2-deficient DC progenitors. In addition, GATA2 was found to bind an enhancer element 190-kb downstream region of Gata3, and a reporter assay exhibited significantly reduced luciferase activity after adding this enhancer region to the Gata3 promoter, which was recovered by GATA sequence deletion within Gata3 +190. These results suggest that GATA2 plays an important role in cell fate specification toward the myeloid versus T lymphocyte lineage by regulating lineage-specific transcription factors in DC progenitors, thereby contributing to DC differentiation.
2016-05-31 | E-GEOD-82044 | biostudies-arrayexpress
Project description:Transcriptional profiling of mouse comparing in vitro-derived DC progenitors from control and Gata2 conditional knockout mice. Two-condition experiment, Control DCs vs. G2 Knockout DCs. Biological replicates: 4 control, 3 Gata2 knockout, independently grown and harvested. One replicate per array.
2016-05-31 | GSE82044 | GEO
Project description:Transcriptional Profiling of Neuronal APP/APLP2 Double-Conditional Knockout Mice.
2015-07-16 | E-GEOD-48622 | ExpressionAtlas
Project description:Gene expression change in Gata2 conditional knockout mice adult renal collecting duct
| PRJNA228968 | ENA
Project description:RNA sequencing (RNA-SEQ) of Dnmt1 and Dnmt3a conditional knockout mice
2019-05-14 | E-GEOD-58261 | ExpressionAtlas
Project description:The homeostatic control mechanism of dendritic cells (DCs), including pDCs, cDC1s and cDC2s, is not fully elucidated. Transcriptome profiling of wildtype and TRIM33 conditional knockout mice revealed a key role of TRIM33 in maintaining the homeostasis of all DC subsets.
2024-06-01 | GSE193183 | GEO
Project description:Transcription profiling of bone marrow hematopoietic stem cells and myeloid progenitors from mice with conditional deletion of Forkhead O (FoxO) family of transcription factors
2014-07-03 | E-GEOD-6623 | ExpressionAtlas
Project description:Gene expression profile of retina and pineal gland of NeuroD1 conditional knockout mice
2015-09-22 | E-GEOD-35396 | ExpressionAtlas
Project description:Transcription profiling by array of CREM-knockout mice
2015-10-22 | E-MEXP-582 | ExpressionAtlas
Project description:Transcription profiling by array of mouse LPS and ATP simulated dendritic cells isolated from microRNA-155 knockout mice or wild-type controls
2014-07-03 | E-MTAB-2608 | ExpressionAtlas