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Mycobacterium

ABSTRACT: Mycobacterium leprae and Mycobacterium lepromatosis from red quirrels

PROVIDER: PRJNA325727 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR3672740.fastq.gz	Fastqsanger.gz
	SRR3672750.fastq.gz	Fastqsanger.gz
	SRR3672741.fastq.gz	Fastqsanger.gz
	SRR3672751.fastq.gz	Fastqsanger.gz
	SRR3672742.fastq.gz	Fastqsanger.gz

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Transcriptome analysis in Mycobacterium leprae (M. leprae)-infected granuloma of BALB/c nu/nu mice

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Profiling of Gene expression in Mycobacterium leprae (M. leprae)-infected footpad of BALB/c nu/nu mice

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Profiling of Gene expression in Mycobacterium leprae (M. leprae)-infected footpad of BALB/c nu/nu mice

Project description:Our goal is to understand the mechanism of granuloma formation in molecular level using Mycobacterium leprae (M. leprae)-infected footpads. One-condition experiment, M. leprae non-infected footpads (control) vs. M. leprae infected footpads (sample). Biological replicates: 6 control, 6 (sample), independently grown and harvested from isolator. One replicate per array.

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Project description:Mycobacterium leprae, the causative agent of leprosy, an obligate intracellular pathogen has the ability to survive and grow for extended periods within phagocytes and Schwann cells. M. leprae genome analysis predicts a highly degraded genome resulting in a significant loss of its genomic coding capacity. Detailed dynamics of carbon sources for energy utilization and growth of M. leprae is unclear. This study, therefore, presents M. leprae transcriptome during in vivo growth and ex vivo stationary phases, and explores metabolic pathways relevant to its growth from global gene expression data. This report provides a glimpse of some of M. leprae nutritional requirements for growth, which most likely, needs to be supplemented, in an axenic growth media.

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Project description:A case of Mycobacterium leprae / Mycobacterium lepromatosis infection in Nuevo Leon, Mexico

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Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus

Project description:Mineralised dental plaque (calculus) has proven to be an excellent source of ancient biomolecules. In this study we present a Mycobacterium leprae genome (6.6-fold), the causative agent of leprosy, recovered via shotgun sequencing of 16th century human dental calculus from an individual from Trondheim, Norway. Moreover, ancient mycobacterial peptides were retrieved via mass spectrometry-based proteomics, further validating the presence of the pathogen. M. leprae can readily be detected in the oral cavity and associated mucosal membranes, which likely contributed to it being incorporated into this individual’s dental calculus. This individual showed some possible, but not definitive, evidence of skeletal lesions associated with early stage leprosy. This study is the first known example of successful multi-omics retrieval of M. leprae from archaeological dental calculus. Furthermore, we offer new insights into dental calculus as an alternative sample source to bones or teeth for detecting and molecularly characterizing M. leprae in individuals from the archaeological record.

2020-10-19 | PXD017836 | Pride

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