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Saccharomyces cerevisiae

ABSTRACT: Transcript profiling of WT and lsm1∆ Saccharomyces cells before and after 1, 2 and 4 hours of hydroxyurea treatment (200 mM)

PROVIDER: PRJNA326236 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3696510.fastq.gz	Fastqsanger.gz
	SRR3696520.fastq.gz	Fastqsanger.gz
	SRR3696511.fastq.gz	Fastqsanger.gz
	SRR3696521.fastq.gz	Fastqsanger.gz
	SRR3696512.fastq.gz	Fastqsanger.gz

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Saccharomyces cerevisiae

Project description:Transcript profiling of Saccharomyces cerevisiae cells overexpressing YOX1 before and after 2 and 4 hours of hydroxyurea (HU) treatment (200 mM)

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CEA SGF:E00013#yap1 aft W303a HU response

Project description:Analysis of the response to hydroxyurea in a yeast aft1 mutant strain compared to wild-type strain (BY4741 or BY4742 backgrounds). Cells were grown in YPD rich medium containong 200 mM hydroxyurea (HU) for 2 hours. Analysis of the response to hydroxyurea in a yeast aft1aft2 mutant strain (Y18aft2d) compared to wild-type strain (CM3260). Cells were grown in YPD rich medium containing 200 mM hydroxyurea (HU) for 2 hours. Gene expression changes due to the aft1 mutation were also analysed in absence of HU (BY4741 background). Analysis of mid-term response to hydroxyurea in a yeast wild-type strain (W303a). Cells were grown in YPD rich medium containing 200 mM hydroxyurea (HU) for 2 hours. Gene expression changes were analysed compared to the same strain grown in the same medium without HU. Keywords: repeat sample

2005-12-01 | GSE1914 | GEO

CEA_SGF:E00013#yap1_HU_response

Project description:Analysis of the response to hydroxyurea in a yeast yap1 mutant strain compared to wild-type strain (BY4741 or BY4742 backgrounds). Cells were grown in YPD rich medium containing 200 mM hydroxyurea (HU) for 2 hours. Keywords: repeat sample

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CEA_SGF:E00016#HU_response_W303a

Project description:Analysis of mid-term response to hydroxyurea in a yeast wild-type strain (W303a). Cells were grown in YPD rich medium containing 200 mM hydroxyurea (HU) for 2 hours. Gene expression changes were analysed compared to the same strain grown in the same medium without HU. Keywords: repeat sample

2005-12-01 | GSE1943 | GEO

Saccharomyces cerevisiae: DSB mapping by Break-chip in a two-color experiment (G1 control vs. S phase samples)

2015-01-12 | GSE64446 | GEO

CEA_SGF:E00013#yap1_aft_W303a_HU_response

2010-06-06 | E-GEOD-1914 | biostudies-arrayexpress

CEA_SGF:E00015#aft_HU_response

Project description:Analysis of the response to hydroxyurea in a yeast aft1aft2 mutant strain (Y18aft2d) compared to wild-type strain (CM3260). Cells were grown in YPD rich medium containing 200 mM hydroxyurea (HU) for 2 hours. Gene expression changes due to the aft1 mutation were also analysed in absence of HU (BY4741 background). Keywords: repeat sample

2005-12-01 | GSE1942 | GEO

CEA_SGF:E00014#HU_response_long-term

Project description:Analysis of long-term response to hydroxyurea in a yeast wild-type strain (CDY59). Cells were grown in YPD rich medium containong 200 mM hydroxyurea (HU) during the indicated time (1, 2, 4 or 6 hours). Gene expression changes were analysed compared to the same strain grown in the same medium without HU. Keywords: time-course

2005-12-01 | GSE1915 | GEO

Saccharomyces cerevisiae: DSB mapping by Break-chip in a two-color experiment (G1 control vs. S phase samples)

Project description:Break-chip (microarray-based double strand break mapping) analysis of mec1 cells recovering from 200 mM hydroxyurea in the presence or absence of 0.8 micromolar bathophenanthroline sulfonate (BPS). We asked if the presence of an iron chelator, BPS, during cell recovery from transient exposure to 200 mM hydroxyurea changes the global patterns of DNA double strand breaks (DSBs). We used a yeast checkpoint mutant, mec1, which has been shown to produce DSBs at replication forks after hydroxyurea was removed from the cell culture. We synchronously released cells from the G1/S transition into S phase in the presence of 200 mM hydroxyurea. After 1h treatment, the drug was removed and cells were allowed to recover in fresh medium for 1h in the presence or absence of 0.8 micromolar BPS. Recover (R) samples, R-1h-BPS and R-1h+BPS, as well as the G1 control samples were collected. Break-chip analysis was performed as previously described (Feng et al., G3(Bethesda) 2011 Oct;1(5):327-35. doi: 10.1534/g3.111.000554).

2015-01-12 | E-GEOD-64446 | biostudies-arrayexpress

CEA_SGF:E00013#yap1_HU_response

2005-11-30 | E-GEOD-1941 | biostudies-arrayexpress