Project description:Gene expression profile of treatment with FABP4 or FABP5 in ADSC and 233A cells was examined. ADSC or 233A cells were treated with and without 1 µM recombinant FABP4 or FABP5.
Project description:Analysis of 20S proteasome in various tissue, adipose derived stem cells(ADSC) or HeLa cells treated with interferon-gamma. This results are compared with LC-SRM results obtained on the same samples. Global proteomics on ADSC amplified in 5% or 20% O2.
Project description:Transcriptional activity was identified in all categories of genes expressed by ADSC, including collagens, ECM and basement membrane constituents, adhesion molecules involved in cell-cell and cell-matrix interactions, and proteases involved in degradation of the ECM and their inhibitors. Importantly, it was observed that ADSC synthesize and secrete all three collagen VI chains, suggesting suitability of ADSC for collagen VI congenital muscular dytrophy treatment. We developed a procedure for isolation of human stem cells from adipose layer of neonatal foreskin skin. The adipose-derived stem cells (ADSC) were examined for expression of extracellular matrix (ECM) and related genes using gene expression array analysis.
Project description:The aim of this study is to characterize how the extracellular matrix secreted by adipose-derived stem cells (ADSC) during osteogenesis affects the differentiation process. Specifically, ADSC undergo osteogenesis by following similar maturational phases as bone marrow-derived stem cells. However, it is unclear how the differentiation process is the same and how it differs. We first focused on ADSC behavior by analyzing whole transcriptome changes in response to osteogenic media supplements added into the tissue culture medium. We then developed osteogenic differentiation expression profiles for the ADSC and identify key genes and pathways that serve as an osteogenesis signature. This expression signature acted as a template for comparison of how extracellular matrix (ECM) affected ADSC differentiation. We studied ADSC induced to differentiate on ECM isolated from day 16 in the differentiation process (the midpoint in osteogenesis) as well as ECM from day 11 in the differentiation process. Ultimately, we aim to dissect the relationship between cells grown on ECM as an in vitro growth substrate and cells grown on tissue culture plastic; both in the presence of osteogenic supplements. This study, will allow us to determine the extent to which ECM affects the differentiation process.
Project description:Alveolar macrophages (AMs) are crucial for pulmonary defense and immune regulation. Exosomes from adipose-derived mesenchymal stem cells (ADSC-Exos) have emerged as a promising therapy for inflammatory lung diseases by modulating immune responses. This study investigates the ability of ADSC-Exos to alleviate inflammation in acute lunag injury (ALI), a condition often triggered by sepsis and characterized by lung permeability and respiratory failure.
Project description:We have used RNA-sequencing on six different proliferating cell lines consisting of normal human dermal fibroblasts (NHDF), normal human epidermal keratinocytes (NHEK), pericytes (PC), human microvADSCular blood endothelial cells (HMEC), lymphatic endothelial cells (LEC) and adipose derived stem cells (ADSC), subjected to different doses of radiotherapy.
Project description:The aim of this study was to elucidate the role of the adipose tissue-derived stem cell (ADSC) secretome on NK cell activity. To elucidate the molecular mechanisms involved, we used mRNA sequencing to profile the transcriptional expression of human blood NK cells from 6 donors.
Project description:The purpose of this study was to evaluate the transcriptomics changes in adipose derived mesenchymal stem cells (ADSC) and an in vitro model of umbilical cord blood cells (UCB) exposed to biosimilar and innovative G-CSF (Granulocyte-colony stimulating factor) for the comparison of molecular targets impacted by both drugs.
Project description:ADSCs are a new type of MSC that is typically abundant in individuals. Numerous investigations reported that adipose-derived stem cell (ADSC) transplantation could ameliorate the structure and function of injured tissues. However, their protective role in premature ovarian failure remains obscure. The aim of this study was to explore the therapeutic efficacy of ADSC transplantation for chemotherapy-induced ovarian damage and microarray analyses were used to assess gene related to ovarian function.
