Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA326503 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We investigated the hormetic effects of prenatal hyperbaric normoxia exposure on Drosophila healthspan related to molecular defense mechanisms. HN exposure had no disruptive effect on developmental rate or adult body weight. However, lifespan was clearly enhanced, as was resistance to oxidative and heat stress. In addition, levels of reactive oxygen species were significantly decreased and motor performance was increased. Furthermore, to determine the hormetic mechanism underlying these phenotypic and molecular changes, we performed a genome-wide profiling in HN-exposed and control flies. Genes encoding chitin metabolism were highly up-regulated in both sex, which could possibly serve to scavenge free radicals. These results identify prenatal HN exposure as a potential hormetic factor that may improve longevity and healthspan by enhancing defense mechanisms in Drosophila.
2017-02-09 | GSE84069 | GEO
Project description:Drosophila melanogaster transcriptomics and epigenomics for oxidative stress response
| PRJNA655140 | ENA
Project description:A study evaluating the effect of stress resistance selection of Drosophila melanogaster. Abstract Here, we report a detailed analysis of changes in gene expression in Drosophila melanogaster selected for multiple eological relevant environmental stress resistance traits. We analyzed females from three biological replicates from seven selection regimes and one control regime using whole genome gene expression arrays. Replicated selection lines were selected for resistance to acute heat survival, high temperature knock down, constant 30°C during development, cold shock survival, desiccation and starvation, respectively. Additionally, a set of replicated lines was selected for increased longevity. When compared to gene expression profiles of control lines, we were able to detect consistent selection responses at the transcript level in each specific selection regime and also found a group of differentially expressed genes that were generally changed among all selected lines. Replicated selection lines clustered together, i.e. showed similar changes in gene expression (compared to controls) and thus showed that 10 generations of artificial selection gives a clear signal among gene expression profiles. The changes in gene expression in lines selected for increased longevity, desiccation and starvation resistance, respectively, showed high similarities. Cold resistance selected lines showed little differentiation from controls. Different methods of heat selection (heat survival, heat knock down and constant 30°C) showed little similarity verifying that different mechanism are involved in high temperature adaptation. The direction of change in gene expression in the selected lines showed a consistent pattern for each selection regime. For most selection regimes and in the comparison of all selected lines and controls exclusively up- or down regulation of gene expression among significant differentially expressed genes was found. The different responses to selection expressed in individual selection regimes and among all selected lines indicate that we have identified genes involved in stress specific and general stress response mechanisms. Keywords: control versus selected
2007-04-16 | GSE6558 | GEO
Project description:Epigenetic alteration is a hallmark of aging, but it remains unclear if perturbation of transcription machineries may affect longevity. NELF-mediated pausing of RNA polymerase II (RNAPII) constitutes an important step in transcription regulation. We found that halving NELF-A level in heterozygous mutants or via neuronal-specific depletion of NELF-A improves locomotor activity, stress resistance and lifespan of Drosophila significantly. Mechanistically, lower NELF-A releases paused RNAPII to facilitate productive transcription of the heat-shock protein (Hsp) genes. Elevated HSPs attenuate the level of protein aggregation, reactive oxidative species, DNA damage and systemic inflammation in the brains of NELF-A depleted flies. This protection is conserved in human cells where NELF-A depletion confers improved resistance against oxidative stress. Reduced oxidative DNA damage allows the maintenance of H3K9me2-enriched heterochromatin, which represses the activation of specific retrotransposons during aging. Therefore, by regulating transcription of Hsp genes in the brains, NELF-A controls multiple aspects of aging in Drosophila.
2020-11-30 | GSE155940 | GEO
Project description:A study evaluating the effect of stress resistance selection of Drosophila melanogaster. Abstract; Here, we report a detailed analysis of changes in gene expression in Drosophila melanogaster selected for multiple ecologically relevant environmental stress resistance traits. We analyzed females from three biological replicates from seven selection regimes and one control regime using whole genome gene expression arrays. Replicated selection lines were selected for resistance to acute heat survival, high temperature knock down, constant 30 degress C during development, cold shock survival, desiccation and starvation, respectively. Additionally, a set of replicated lines was selected for increased longevity. When compared to gene expression profiles of control lines, we were able to detect consistent selection responses at the transcript level in each specific selection regime and also found a group of differentially expressed genes that were generally changed among all selected lines. Replicated selection lines clustered together, i.e. showed similar changes in gene expression (compared to controls) and thus showed that 10 generations of artificial selection gives a clear signal among gene expression profiles. The changes in gene expression in lines selected for increased longevity, desiccation and starvation resistance, respectively, showed high similarities. Cold resistance selected lines showed little differentiation from controls. Different methods of heat selection (heat survival, heat knock down and constant 30 degrees C) showed little similarity verifying that different mechanism are involved in high temperature adaptation. The direction of change in gene expression in the selected lines showed a consistent pattern for each selection regime. For most selection regimes and in the comparison of all selected lines and controls exclusively up- or down regulation of gene expression among significant differentially expressed genes was found. The different responses to selection expressed in individual selection regimes and among all selected lines indicate that we have identified genes involved in stress specific and general stress response mechanisms. Experiment Overall Design: gene expression was measured in biological triplicates of control flies and flies selected for resistance to various stressors.
2009-02-07 | E-GEOD-6558 | biostudies-arrayexpress
Project description:Gene expression changes in response to aging compared to heat stress, oxidative stress and ionizing radiation in Drosophila melanogaster
| PRJNA179449 | ENA
Project description:Broadly distributed species must cope with diverse and changing environmental conditions, including various forms of stress. Cosmopolitan populations of Drosophila melanogaster are more tolerant to oxidative stress than those from the species’ ancestral range in sub-Saharan Africa, and the degree of tolerance is associated with an insertion/deletion polymorphism in the 3’ untranslated region of the Metallothionein A (MtnA) gene that varies clinally in frequency. We examined oxidative stress tolerance and the transcriptional response to oxidative stress in cosmopolitan and sub-Saharan African populations of D. melanogaster, including paired samples with allelic differences at the MtnA locus. We found that the effect of the MtnA polymorphism on oxidative stress tolerance was dependent on the genomic background, with the deletion allele increasing tolerance only in a northern, temperate population. Genes that were differentially expressed under oxidative stress included MtnA and other metallothioneins, as well as those involved in glutathione metabolism and other genes known to be part of the oxidative stress response or the general stress response.
2022-01-11 | GSE174813 | GEO
Project description:The aim of this study was to use unbiased transcriptomic analysis to characterize new traits that may explain differences in longevity between short- and long-lived wild-type backgrounds of Drosophila melanogaster – Dahomey and Oregon R, respectively. For the experiment we chose young flies (10 days old) to capture the difference in basal gene expression related to the genotype rather than to age-dependent functional decline. As a source for RNA extraction we used heads and thoraxes (combined) as tissues the most sensitive to aging. The expression of 3939 genes was changed (nearly 26% of the transcriptome, p-value < 0.05), with 1970 being upregulated and 1969 genes being downregulated in the Dahomey background compared to Oregon R. We found that young short-lived Dahomey flies have the traits previously associated with shorten lifespan such as increased lipo-oxidative stress, increased Tor signaling and loss of proteostasis and mitochondrial complex I activity. We hypothesized that all these characteristics are caused by an increase in octopamine signaling that promotes foraging behavior even under laboratory conditions where nutrients are in excess. Our results highlight the importance of controlling the genetic background in aging studies as well as interrogating several different pathways before making conclusions about what causes differences in longevity between different groups or individuals.
2018-06-27 | GSE101465 | GEO
Project description:During oxidative stress, reactive oxygen species (ROS) can modify and damage cellular proteins. In particular, the thiol groups of cysteine residues can undergo reversible or irreversible oxidative post-translation modifications (PTMs). Identifying the redox-sensitive cysteines on a proteome-wide scale can provide insight into those proteins that act as redox sensors or become irreversibly damaged upon exposure to high levels of ROS. Aging is accompanied by oxidative stress, and oxygen-rich tissues such as the eye are particularly vulnerable because of its high energy demand and generation of ROS byproducts, which increases the risk for ocular disease. In this study, we profiled the redox proteome of the aging Drosophila eye to identify cysteine residues that are modified by age-associated ROS.
2023-04-27 | PXD036110 | Pride