Project description:Members of the genus Acinetobacter drag attention due to their importance in microbial pathology and biotechnology. OmpA is a porin with multifaceted functions in different species of Acinetobacter. In this study we identified this protein in Acinetobacter sp. SA01, an efficient phenol degrader strain, in different cellular and sub-cellular compartments (such as OM, OMV, biofilm and extracellular environment). Differential expression of proteins, including OmpA, under two conditions of phenol and ethanol supplementation was assessed using shotgun proteomics.
Project description:In recent years, the Gram-negative bacterium Acinetobacter baumannii has garnered considerable attention for its unprecedented capacity to rapidly develop resistance to antibacterial therapeutics. This is coupled with the seemingly epidemic emergence of new hyper-virulent strains. Although strain-specific differences for A. baumannii isolates have been well described, these studies have primarily focused on proteinaceous factors. At present, only limited publications have investigated the presence and role of small regulatory RNA (sRNA) transcripts. Herein, we perform such an analysis, describing the RNA-seq-based identification of 78 A. baumannii sRNAs in the AB5075 background. Together with six previously identified elements, we include each of these in a new genome annotation file, which will serve as a tool to investigate regulatory events in this organism. Our work reveals that the sRNAs display high expression, accounting for >50 % of the 20 most strongly expressed genes. Through conservation analysis we identified six classes of similar sRNAs, with one found to be particularly abundant and homologous to regulatory, C4 antisense RNAs found in bacteriophages. These elements appear to be processed from larger transcripts in an analogous manner to the phage C4 molecule and are putatively controlled by two further sRNAs that are strongly antisense to them. Collectively, this study offers a detailed view of the sRNA content of A. baumannii, exposing sequence and structural conservation amongst these elements, and provides novel insight into the potential evolution, and role, of these understudied regulatory molecules. This study is based on the annotation of novel sRNAs on basis of an Acinetobacter baumannii RNA sequencing dataset. Each sample was generated by pooling three independent biological replicate RNA preps
Project description:We performed RNAseq for gene expression analysis for six strains of Acinetobacter Baumannii isolated from blood samples (defined as strains 1, 2, 3, 4 and 6) of patients hospitalized at the University Hospital \\"San Giovanni di Dio e Ruggi d'Aragona\\" (Salerno, Italy)
Project description:This model is part of a collection comprising eight strain-specific genome-scale metabolic models of five different Acinetobacter baumannii strains, seven of which have been previously published and were now collected and carefully curated. All models were checked with the SBML validator, MEMOTE, and FROG. The covered strains include Acinetobacter baumannii AYE, AB0057, ATCC 19606, ATCC 17978, and AB5075.
Project description:Studies of expression of mechanims of defense of the Acinetobacter sp.5-2Ac.02 from airborne hospital environment under stress conditions, such as SOS response (ROS response, heavy metals resistant mechanisms, peptides), as well as Quorum network (acetoin cluster and aromatics biodegradation cluster). Characterization functional of AcoN-like as negative regulator protein from acetoin cluster in Acinetobacter spp. Strains
Project description:Collimonas is a genus of soil bacteria which comprises three recognized species: C. fungivorans, C. pratensis and C. arenae. The bacteria belonging to this genus share the ability to lyse chitin (chitinolysis) and feed on living fungal hyphae (mycophagy), but they differ in colony morphology, physiological properties and antifungal activity. In order to gain a better insight into the genetic background underlying this phenotypic variability of collimonads, we investigated the variability in the genomic content of five strains representing the three formally recognized Collimonas species. The genomic content of four test strains was hybridized on an array representing the reference strain C. fungivorans Ter331.
Project description:This model is part of a collection comprising eight strain-specific genome-scale metabolic models of five different Acinetobacter baumannii strains. Seven of these models have been previously published and were now collected and carefully curated. All models were checked with the SBML validator, MEMOTE, and FROG. The covered strains include Acinetobacter baumannii AYE, AB0057, ATCC 19606, ATCC 17978, and AB5075.
Project description:This model is part of a collection comprising eight strain-specific genome-scale metabolic models of five different Acinetobacter baumannii strains. Seven of these models have been previously published and were now collected and carefully curated. All models were checked with the SBML validator, MEMOTE, and FROG. The covered strains include Acinetobacter baumannii AYE, AB0057, ATCC 19606, ATCC 17978, and AB5075.
Project description:This model is part of a collection comprising eight strain-specific genome-scale metabolic models of five different Acinetobacter baumannii strains. Seven of these models have been previously published and were now collected and carefully curated. All models were checked with the SBML validator, MEMOTE, and FROG. The covered strains include Acinetobacter baumannii AYE, AB0057, ATCC 19606, ATCC 17978, and AB5075.