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Thunnus maccoyii

ABSTRACT: Genome sequencing and assembly of southern bluefin and other tuna species

PROVIDER: PRJNA327759 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3932110_1.fastq.gz	Fastqsanger.gz
	SRR3932110_2.fastq.gz	Fastqsanger.gz
	SRR3932105_1.fastq.gz	Fastqsanger.gz
	SRR3932105_2.fastq.gz	Fastqsanger.gz
	SRR3932106_1.fastq.gz	Fastqsanger.gz

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Thunnus orientalis

Project description:Pacific bluefin tuna genome sequencing

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Microarray gene expression profiles from temperature acclimated atrial, ventricular compact and spongy tissues of Pacific bluefin tuna Thunnus orientalis

Project description:In this study, we examined the transcriptomic responses to temperature acclimation (14oC, 20oC, and 25oC) in atrial and ventricular tissues of Pacific bluefin tuna (PBFT). A global gene expression analysis using a bluefin tuna-specific microarray indicated profound changes in expression of genes associated with energy metabolism, protein turnover, cellular stress response, oxidative stress and apoptosis. A principal component analysis revealed tissue-specific transcriptomic responses to temperature, with atrium at 25oC showing the greatest variation. Overall transcriptomic data suggests that PBFT can optimize cardiac function in the cold by acclimating to 14oC. Capacity to acclimate to colder temperatures potentially underlies this species ability to expand its vertical and horizontal thermal niche and migrate to colder oceans at high latitudes. In contrast, the cardiac phenotype of 25oC acclimated fish infers that PBFT hearts struggle to maintain cellular homeostasis and are subjected to programmed cell death. The goal of this study was to compare transcriptomic response to cold and warm temperature acclimations across cardiac tissues in Pacific bluefin tuna. Fish (n=4) were acclimated to 14C, 20C and 25C, and RNA was extracted from atrial, ventricular compact and spongy tissues. Experimental samples were hybridyzed against a reference pool that contained a mix of RNA from every sample.

2012-03-31 | E-GEOD-36886 | biostudies-arrayexpress

Development and validation of a mixed-tissue oligo microarray for Atlantic bluefin tuna, Thunnus thynnus (Linnaeus, 1758)

Project description:The largest of the tuna species, Atlantic bluefin tuna, Thunnus thynnus (Linnaeus, 1758), inhabits the North Atlantic Ocean and the Mediterranean Sea and is considered to be an endangered species, largely through overfishing. Thus, the development of aquaculture practices independent of wild resources can provide an important contribution towards ensuring security and sustainability of this species in the longer-term. In order to provide a resource for ongoing studies, we have used 454 pyrosequencing technology to sequence a mixed-tissue normalized cDNA library, derived from adult individuals. Transcript sequences were used to develop a novel 15K Agilent oligo microarray for T. thynnus and comparative tissue gene expression profiles were inferred for gill, heart, liver, ovaries and testes.

2015-08-10 | E-MTAB-3412 | biostudies-arrayexpress

Microarray gene expression profiles from temperature acclimated atrial, ventricular compact and spongy tissues of Pacific bluefin tuna Thunnus orientalis

2012-04-01 | GSE36886 | GEO

Docosahexaenoic acid (DHA); a driving force regulating gene expression in bluefin tuna (Thunnus thynnus) larvae development.

Project description:This study elucidated the role of DHA-modulated genes in the development and growth of bluefin tuna (Thunnus thynnus) larvae ingesting increasing levels of DHA in their rotifer prey. The effect of feeding low, medium, and high rotifer (Brachionus rotundiformis) DHA levels (2.0, 3.6 and 10.9 mg DHA g-1 DW, respectively) was tested on 2-15 days post hatching (dph) bluefin tuna larvae. Larval DHA content markedly (P < 0.05) increased in a DHA dose-dependent manner (1.5, 3.9, 6.1 mg DHA g-1 DW larva, respectively), that was positively correlated with larval prey consumption, and growth (P < 0.05). Gene ontology enrichment analyses of DEGs demonstrated dietary DHA significantly (P < 0.05) affected different genes and biological processes at different developmental ages. The number of DHA up-regulated DEGs was highest in 10 dph larvae (408), compared to 5 (11) and 15 dph fish (34), and were mainly involved in neural and synaptic development in the brain and spinal cord. In contrast, DHA in older 15 dph larvae elicited fewer DEGs but played critical roles over a wider range of developing organs. The emerging picture underscores the importance of DHA-modulated gene expression as a driving force in bluefin tuna larval development and growth.

2024-10-09 | GSE263473 | GEO

Thunnus maccoyii

Project description:RNA-Seq of Southern bluefin tuna (Thunnus maccoyii) gonad cells

| PRJNA288188 | ENA

Thunnus orientalis

Project description:Genome assembly of the Pacific bluefin tuna and re-sequence data analysis

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Project description:Thunnus maccoyii (Southern bluefin tuna)