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Neisseria gonorrhoeae FA19

ABSTRACT: Penicillin-sensitive strain

PROVIDER: PRJNA32793 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR004131.fastq.gz	Fastqsanger.gz
	SRR032792.fastq.gz	Fastqsanger.gz
	SRR156335.fastq.gz	Fastqsanger.gz
	SRR360675_1.fastq.gz	Fastqsanger.gz

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Project description:Penicillin-resistant strain

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Expression data from Neisseria gonorrhoeae (FA19)

Project description:Naturally occurring mtrR mutants of gonococci displaying clinically relevant levels of antibiotic resistance are often isolated from patients and mtrR mutants have been reported to be more fit than the wild type parent strain in a murine vaginal infection model. DNA-binding proteins, such as MtrR, that negatively regulate bacterial efflux pump genes have been considered to be “local” gene regulators, although there is increasing evidence that they can directly or indirectly influence expression of other genes. To define the regulatory properties of MtrR we employed microarray analysis of isogenic MtrR-positive and MtrR-negative gonococci. Keywords: single time point

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Project description:The overall goals and objectives of this study are to investigate the transcriptomics of Neisseria gonorrhoeae using RNA-seq. This work will look at gene expression, start points of transcription, transcriptional termination, and differences between these in different conditions and between strains and growing cultures over time.

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Influence of RppH on the small RNA transcriptome of Neisseria gonorrhoeae MS11

Project description:We assessed the small RNA transcriptome of Neisseria gonorrhoeae strain MS11 in two genetic backgrounds; using wild type cells as well as cells carrying a rppH insertional mutation. It was found that the presence of the RppH enzyme affected both the quantity and length of small RNAs in various chromosomal locations. However, in comparing the two transcriptomes, we found that not all small RNAs were differentially expressed, suggesting that RppH targets only a subset of transcripts.

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RNA-Seq analysis of the Neisseria gonorrhoeae GntR-type transcriptional regulator GdhR

Project description:In this work we have determined by RNA-Seq the whole set of genes regulated by the N. gonorrhoeae GdhR transcriptional regulator (NGO1360 gene in reference strain FA1090). The results showed that GdhR regulates the expression of 2.3% of all the genes in the gonococcal (strain FA19) genome, of which 39 were activated and 11 were repressed. Most of the GdhR-regulated genes lie in the category of fimbrial proteins and membrane antigens or transporters. GdhR was found not be a cryptic regulator since there is an overlap in the set of GdhR-regulated genes between the wild type and a strain expressing gdhR ectopically from the lac promoter. Among the GdhR-regulated genes linked to gonococcal pathogenesis we found that lctP, encoding a unique L-lactate transporter, was the highest of GdhR-repressed genes.

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Transcription profiling of Neisseria gonorrhoeae and Neisseria meningitidis grown at different temperatures

Project description:Wild type Neisseria gonorrhoea strain FA1090 and N. meningitidis strain MC58 were grown on normal GC plate at either 35 degree celsius (for control samples) or 40 degree celsius (for test samples)

2007-11-20 | E-MEXP-590 | biostudies-arrayexpress

Neisseria gonorrhoeae drg::cm versus wild type and Neisseria gonorrhoeae drg::dam versus wild type

Project description:Transcriptional profiling of N. gonorrhoeae comparing wild type cells to cells with inactivated by chloramphenicol cassette (cm) dam replacing gene (drg) or wild type cells comparing to cells with inserted dam gene. The Goal was to study the role of drg or dam presence in overall expression profile.

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Neisseria gonorrhoeae

Project description:The MisR regulon in Neisseria gonorrhoeae strain FA19

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The MisR regulon in Neisseria gonorrhoeae strain FA19

Project description:Whole transcriptome analysis of N. gonorrhoeae FA19 and isogenic NGEG_00293 (misR) mutant using RNA-Seq (note that NGO0177 is the misR ORF designation in mapping strain FA1090) Examination of total transcriptomes in N. gonorrhoeae FA19 WT and an FA19 misR::kan isogenic mutant to determine the regulatory impact of the MisR response regulator on cells grown under laboratory conditions. Illumina HiSeq-2000 next generation sequencing was used to sequence the transcriptomes of each strain. Reads were mapped against the N. gonorrhoeae FA1090 genome (NCBI accession number NC_002946) because at the time this experiment was run the FA19 genome was incomplete.

2014-05-16 | E-GEOD-50184 | biostudies-arrayexpress

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