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Protosalanx hyalocranius

ABSTRACT: Protosalanx hyalocranius Raw sequence reads

PROVIDER: PRJNA328051 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR3747720_1.fastq.gz	Fastqsanger.gz
	SRR3747720_2.fastq.gz	Fastqsanger.gz
	SRR3747721_1.fastq.gz	Fastqsanger.gz
	SRR3747721_2.fastq.gz	Fastqsanger.gz
	SRR3747722_1.fastq.gz	Fastqsanger.gz

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Protosalanx hyalocranius

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Protosalanx hyalocranius

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Protosalanx hyalocranius

Project description:Whole genome re-sequencing reads for Protosalanx hyalocranius

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Protosalanx hyalocranius isolate:A1

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Protosalanx chinensis

Project description:Protosalanx chinensis Raw sequence reads

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Whole genome sequencing of Chinese clearhead icefish, Protosalanx hyalocranius.

Project description:Background:Chinese clearhead icefish, Protosalanx hyalocranius , is a representative icefish species with economic importance and special appearance. Due to its great economic value in China, the fish was introduced into Lake Dianchi and several other lakes from the Lake Taihu half a century ago. Similar to the Sinocyclocheilus cavefish, the clearhead icefish has certain cavefish-like traits, such as transparent body and nearly scaleless skin. Here, we provide the whole genome sequence of this surface-dwelling fish and generated a draft genome assembly, aiming at exploring molecular mechanisms for the biological interests. A total of 252.1 Gb of raw reads were sequenced. Subsequently, a novel draft genome assembly was generated, with the scaffold N50 reaching 1.163 Mb. The genome completeness was estimated to be 98.39 % by using the CEGMA evaluation. Finally, we annotated 19?884 protein-coding genes and observed that repeat sequences account for 24.43 % of the genome assembly. We report the first draft genome of the Chinese clearhead icefish. The genome assembly will provide a solid foundation for further molecular breeding and germplasm resource protection in Chinese clearhead icefish, as well as other icefishes. It is also a valuable genetic resource for revealing the molecular mechanisms for the cavefish-like characters.

| S-EPMC5530312 | biostudies-literature

Protosalanx chinensis

Project description:Protosalanx chinensis overview

| PRJNA605057 | ENA

Female-specific genomic regions and molecular sex identification of the clearhead icefish (Protosalanx hyalocranius).

Project description:BackgroundThe clearhead icefish, Protosalanx hyalocranius, is an economically important fishery species in China. Since 1980s, P. hyalocranius was widely introduced into lakes and reservoirs of northern China for aquaculture. However, the lack of a rapid and cost-effective sex identification method based on sex specific genetic markers has hindered study on sex determination mechanisms and breeding applications.ResultsFemale-specific genomic regions were discovered by comparing whole genome re-sequencing data of both males and females. Two female-specific genomic regions larger than 50 bp were identified, and one (598 bp) contained a putative FOXI gene, which was paralogous to another FOXI gene with sex-associated SNPs. The two FOXI sequences displayed significant length difference with nine deletions of total length of 230 bp. This deletion-type structural variation could be easily and efficiently detected by traditional PCR and agarose gel electrophoresis with one 569 bp band for males and two bands (569 and 339 bp) for females, which were validated in 50 females and 40 males with known phenotypic sexes.ConclusionsThe results provided structural genomic evidence for the ZZ/ZW sex determination system in P. hyalocranius discovered in our previous study with association analysis of SNPs. Moreover, the female-specific markers and rapid and cost-effective PCR-based genetic sex identification method should have applications in further studies of sex determination mechanism for this species.

| S-EPMC8254354 | biostudies-literature

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