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Benzotriazoles reactivate latent HIV-1 through inactivation of STAT5 SUMOylation

ABSTRACT: Benzotriazoles reactivate latent HIV-1 through inactivation of STAT5 SUMOylation

PROVIDER: PRJNA328275 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3802511_1.fastq.gz	Fastqsanger.gz
	SRR3802511_2.fastq.gz	Fastqsanger.gz
	SRR3802521_1.fastq.gz	Fastqsanger.gz
	SRR3802521_2.fastq.gz	Fastqsanger.gz
	SRR3802513_1.fastq.gz	Fastqsanger.gz

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Benzotriazoles reactivate latent HIV-1 through inactivation of STAT5 SUMOylation (ChIP-Seq)

Project description:We screened for small molecules that reactivated latent HIV-1 and identified benzotriazoles as latency-reducing agents. Here, we characterize the effects of HODHBt on gene expression in cultured T cells from three donors by polyA RNA-Seq and on STAT5A occupancy of the HIV-1 LTR promoter by ChIP-Seq. These and other results demonstrate that benzotriazoles block SUMOylation of phosphorylated STAT5, prolonging its transcriptional activity.

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Benzotriazoles Reactivate Latent HIV-1 through Inactivation of STAT5 SUMOylation.

Project description:The presence of latent HIV-1 in infected individuals represents a major barrier preventing viral eradication. For that reason, reactivation of latent viruses in the presence of antiretroviral regimens has been proposed as a therapeutic strategy to achieve remission. We screened for small molecules and identified several benzotriazole derivatives with the ability to reactivate latent HIV-1. In the presence of IL-2, benzotriazoles reactivated and reduced the latent reservoir in primary cells, and, remarkably, viral reactivation was achieved without inducing cell proliferation, T cell activation, or cytokine release. Mechanistic studies showed that benzotriazoles block SUMOylation of phosphorylated STAT5, increasing STAT5's activity and occupancy of the HIV-1 LTR. Our results identify benzotriazoles as latency reversing agents and STAT5 signaling and SUMOylation as targets for HIV-1 eradication strategies. These compounds represent a different direction in the search for "shock and kill" therapies.

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Benzotriazoles reactivate latent HIV-1 through inactivation of STAT5 SUMOylation

Project description:This SuperSeries is composed of the SubSeries listed below.

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