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Escherichia coli ATCC 8739

ABSTRACT: Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli

PROVIDER: PRJNA328483 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3825304_1.fastq.gz	Fastqsanger.gz
	SRR3825304_2.fastq.gz	Fastqsanger.gz
	SRR3825305_1.fastq.gz	Fastqsanger.gz
	SRR3825305_2.fastq.gz	Fastqsanger.gz
	SRR3825306_1.fastq.gz	Fastqsanger.gz

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Escherichia coli ATCC 8739

Project description:A novel point mutation in RpoB improves osmotolerance and succinic acid production in Escherichia coli

| PRJNA331108 | ENA

Differential gene expression of Escherichia coli ATCC 8739 in strawberry nectar after thermal and non-thermal treatments

Project description:Red fruits are valued for their vitamin C and polyphenol content, but traditional heat preservation methods used in juice and nectar production can significantly reduce these components. Therefore, alternative non-thermal methods are explored to inactivate foodborne pathogens like Escherichia coli while maintaining the nutritional value. However, knowledge about the effects of these technologies on bacterial cells is limited. This study analyzed differentially expressed genes of E. coli ATCC 8739 inoculated in strawberry nectar after exposure to three treatments with two sets of parameters each, namely thermal treatment, high-pressure processing (HPP), and moderate-intensity pulsed electric field (MIPEF). The highest inactivation efficiency was achieved with HPP at 400 MPa, 1 min, reducing microbial counts by 5.0±0.3 log cfu/mL, and thermal treatment at 60°C, 200 s, achieving a reduction of 4.4±0.2 log cfu/mL, while no inactivation was observed with MIPEF at 6 kV/cm. Transcriptomic analysis showed that thermal and HPP treatments caused similar molecular stress responses in E. coli. In both cases, the most overexpressed genes encoded outer membrane proteins, which may lead to the activation of the envelope stress response. Despite no microbial inactivation was revealed after MIPEF treatment, strong transcriptomic responses were observed, particularly in genes related to membrane integrity and metabolic activity. Numerous overexpressed genes associated with ABC transporters, outer membrane proteins, and lipoproteins were identified, which could increase the strain’s virulence. This study provides insights into the stress response mechanisms induced by conventional and novel treatments. Nevertheless, further research is needed to investigate the long-term effects on bacterial populations.

2024-12-03 | GSE283248 | GEO

Escherichia coli ATCC 8739

Project description:Escherichia coli ATCC 8739 Assembly

| PRJNA495691 | ENA

Escherichia coli ATCC 8739

Project description:Escherichia coli ATCC 8739 Genome sequencing

| PRJNA564864 | ENA

Escherichia coli ATCC 8739

Project description:Escherichia coli ATCC 8739 Raw sequence reads

| PRJNA908231 | ENA

Escherichia coli ATCC 8739

Project description:Escherichia coli Transcriptome: Untreated 1.0%, v/v DMSO treated 2.5%, v/v DMSO treated

| PRJNA777047 | ENA

Viability of Escherichia coli ATCC 8739 in Nutrient Broth, Luria-Bertani Broth and Brain Heart Infusion over 11 Weeks.

Project description:BackgroundEscherichia coli is a widely studied prokaryotic system. A recent study had demonstrated that reduced growth of E. coli after extended culture in Luria-Bertani broth is a result of depletion of fermentable sugars but able to sustain extended cell culture due to the presence of amino acids, which can be utilized as a carbon source. However, this had not been demonstrated in other media. The study aimed to determine the growth and viability of E. coli ATCC 8739 in 3 different media, Nutrient Broth (NB), Brain Heart Infusion (BHI) and Luria-Bertani Broth (LB) over 11 weeks.MethodsGrowth of E. coli ATCC 8739 was determined by optical density. Viability was determined by serial dilution/spread-plate enumeration. After 11 weeks, the media were exhausted by repeated culture. Glucose was added to the exhausted media to determine whether glucose is the growth-limiting factor.ResultsOur results showed that cell density in all 3 media increased to about 1 × 10(9) cells/ml by the end of week 1, from the inoculation density of 2.67 × 10(5) cells/ml, peaked at about 1 × 10(13) cells/ml at week 4, before declining to about 5 × 10(7) cells/ml at week 7. Cell density is highly correlated to genomic DNA content (r(2) = 0.93) but poorly correlated to optical density (r(2)< 0.2). Our results also showed that the spent media were able to support further growth after glucose-supplementation.ConclusionNB, LB and BHI are able to support extended periods of culture and glucose depletion is the likely reason for declining cell growth.

| S-EPMC4477771 | biostudies-literature

Escherichia coli strain:ATCC 8739

Project description:Escherichia coli Transcriptome: Untreated 1.0%, v/v DMSO treated 2.5%, v/v DMSO treated

| PRJNA777030 | ENA

Escherichia coli strain:ATCC 8739

Project description:Investigation of spatial vulnerabilities of Bacterium Escherichia coli genome to spontaneous mutations by molecularly barcoded deep Sequencing

| PRJNA476837 | ENA

Escherichia coli ATCC 8739 isolate:AgNO3 resistant

Project description:Silver resistant Escherichia coli Genome sequencing and assembly

| PRJNA398473 | ENA