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Integrated analyses identify phenotype specific BMP4 signaling in breast cancer

ABSTRACT: Integrated analyses identify phenotype specific BMP4 signaling in breast cancer

PROVIDER: PRJNA329667 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3931130.fastq.gz	Fastqsanger.gz
	SRR3931131.fastq.gz	Fastqsanger.gz
	SRR3931132.fastq.gz	Fastqsanger.gz
	SRR3931133.fastq.gz	Fastqsanger.gz
	SRR3931134.fastq.gz	Fastqsanger.gz

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Integrated analyses identify phenotype specific BMP4 signaling in breast cancer [RNA-seq]

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Integrated analyses identify phenotype specific BMP4 signaling in breast cancer [Dnase-seq]

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Integrated analyses identify phenotype specific BMP4 signaling in breast cancer

Project description:This SuperSeries is composed of the SubSeries listed below.

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Integrated RNA-seq and DNase-seq analyses identify phenotype-specific BMP4 signaling in breast cancer.

Project description:Bone morphogenetic protein 4 (BMP4) plays an important role in cancer pathogenesis. In breast cancer, it reduces proliferation and increases migration in a cell line-dependent manner. To characterize the transcriptional mediators of these phenotypes, we performed RNA-seq and DNase-seq analyses after BMP4 treatment in MDA-MB-231 and T-47D breast cancer cells that respond to BMP4 with enhanced migration and decreased cell growth, respectively.The RNA-seq data revealed gene expression changes that were consistent with the in vitro phenotypes of the cell lines, particularly in MDA-MB-231, where migration-related processes were enriched. These results were confirmed when enrichment of BMP4-induced open chromatin regions was analyzed. Interestingly, the chromatin in transcription start sites of differentially expressed genes was already open in unstimulated cells, thus enabling rapid recruitment of transcription factors to the promoters as a response to stimulation. Further analysis and functional validation identified MBD2, CBFB, and HIF1A as downstream regulators of BMP4 signaling. Silencing of these transcription factors revealed that MBD2 was a consistent activator of target genes in both cell lines, CBFB an activator in cells with reduced proliferation phenotype, and HIF1A a repressor in cells with induced migration phenotype.Integrating RNA-seq and DNase-seq data showed that the phenotypic responses to BMP4 in breast cancer cell lines are reflected in transcriptomic and chromatin levels. We identified and experimentally validated downstream regulators of BMP4 signaling that relate to the different in vitro phenotypes and thus demonstrate that the downstream BMP4 response is regulated in a cell type-specific manner.

| S-EPMC5225521 | biostudies-literature

Homo sapiens

Project description:Integrated analyses identify phenotype specific BMP4 signaling in breast cancer [Dnase-seq]

| PRJNA329671 | ENA

Homo sapiens

Project description:Integrated analyses identify phenotype specific BMP4 signaling in breast cancer [RNA-seq]

| PRJNA329672 | ENA

Compare transcriptomes of BMP4 and BMP4 plus Palo treatment in 2H11 cells

Project description:In order to observe palovarotene modulates BMP4-induced transcriptome,2H11 cells treated with BMP4 or BMP4 plus palovarotene for 48 hours were subjected to RNAseq analyses

2021-03-04 | GSE168157 | GEO

Integrated analyses of translatome and proteome identify the rules of translation selectivity in RPS14-deficient

Project description:Integrated analyses of translatome and proteome identify the rules of translation selectivity in RPS14-deficient

| PRJNA428737 | ENA

Keratin5-cytoskeleton-BMP4 network regulates cell phenotype conversions during cardiac regeneration

Project description:Keratin5-cytoskeleton-BMP4 network regulates cell phenotype conversions during cardiac regeneration

| PRJNA664012 | ENA

Circulating endoglin induces hypertension via the upregulated expression of BMP4

Project description:Endoglin is a membrane glycoprotein primarily expressed by the vascular endothelium and involved in cardiovascular diseases. Upon the proteolytic processing of the membrane-bound protein, a circulating form of endoglin (soluble endoglin, sEng) can be released, and high levels of sEng have been observed in several endothelial-related pathological conditions, where it appears to contribute to endothelial dysfunction. Preeclampsia is a multisystem disorder of high prevalence in pregnant women characterized by the onset of high blood pressure and often associated with increased levels of sEng. Although a pathogenic role for sEng involving hypertension has been reported in several animal models of preeclampsia, the exact molecular mechanisms implicated remain to be identified. To search for sEng-induced mediators, we have analyzed the protein secretome of human endothelial cells in the presence of sEng. We find that sEng induces the expression of BMP4 in endothelial cells, as evidenced by their proteomic signature, gene transcript levels and BMP4 promoter activity. A mouse model of preeclampsia with high sEng plasma levels (sEng+) showed increased levels of BMP4 transcripts in lungs and increased circulating BMP4, compared to those of control animals. In addition, after crossing female wild type with male sEng+ mice, hypertension appears 18 days after mating, precisely coinciding with the appearance of high plasma levels of BMP4. Also, serum levels of sEng and BMP4 are positively correlated in pregnant women with and without preeclampsia. Interestingly, sEng-induced arterial pressure elevation in sEng+ mice was abolished in the presence of the BMP4 inhibitor noggin, suggesting that BMP4 is a downstream mediator of sEng. These results provide a better understanding on the role of sEng in the pathobiology of preeclampsia and other cardiovascular diseases, where sEng levels are increased.

2020-04-23 | PXD016228 | Pride

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