Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression of peripheral blood mononuclear cells from adults with sickle cell disease

ABSTRACT: Gene expression of peripheral blood mononuclear cells from adults with sickle cell disease

PROVIDER: PRJNA330707 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

A Validated Gene Signature from Peripheral Blood Mononuclear Cells in Pulmonary Hypertension Associated with Sickle Cell Disease

Project description:Pulmonary hypertension (PH) is a serious complication of sickle cell disease (SCD) associated with increased mortality. Gene expression profiles of peripheral blood mononuclear cells (PBMC) have been studied in pulmonary arterial hypertension and in SCD. We hypothesized that a PBMC-derived gene signature in SCD patients may be utilized as a PH biomarker. Twenty-seven patients with homozygous SCD underwent transthoracic echocardiography and PBMC isolation. PH was defined as estimated right ventricular systolic pressure (RVSP)>30 mmHg with a peak tricuspid regurgitation velocity (TRV)>2.5m/s. Genome-wide gene expression profiles were correlated against PH severity using RVSP and TRV as surrogates, which yielded 631 potentially dysregulated transcripts. Total RNA was isolated from PBMCs using standard molecular biology protocols without DNA contamination or RNA degradation. Sample processing (e.g., cDNA generation, fragmentation, end labeling, hybridization to Affymetrix GeneChip Human Exon 1.0 ST arrays) was performed per manufacturer’s instructions. A total of 27 African descent American patients with sickle cell disease were included in the microarray analysis.

2012-06-06 | E-GEOD-38528 | biostudies-arrayexpress

Microarray profiling peripheral blood mononuclear cells

Project description:Microarray profiling peripheral blood mononuclear cells

| PRJNA418401 | ENA

A Validated Gene Signature from Peripheral Blood Mononuclear Cells in Pulmonary Hypertension Associated with Sickle Cell Disease

2012-06-07 | GSE38528 | GEO

Gene expression of peripheral blood mononuclear cells from adults with sickle cell disease (UIC cohort)

Project description:Sickle cell disease is associated with systemic complications, many associated with either severity of disease or increased risk of mortality. We sought to identify a circulating gene expression profile whose predictive capacity spanned the spectrum of these poor outcomes in sickle cell disease. The Training cohort consisted of patients with SCD who were prospectively recruited from the University of Illinois. The Testing cohort consisted of a combination of patients prospectively seen at two separate institutions including the University of Chicago and Howard University

2017-09-08 | GSE84632 | GEO

Proteome signatures of inflammatory activated primary human peripheral blood mononuclear cells

Project description:Primary human peripheral blood mononuclear cells were isolated from full blood by standard ficoll centrifugation. Cells were washed and processed immediately.

2012-07-16 | PRD000731 | Pride

Gene expression of peripheral blood mononuclear cells from adults with sickle cell disease (Howard University cohort)

2017-09-08 | GSE84633 | GEO

Gene expression of peripheral blood mononuclear cells from adults with sickle cell disease (University of Chicago cohort)

2017-09-08 | GSE84634 | GEO

Metabolic and Proteomic Changes in Sickle Cell Disease and thalassemia Mouse Splenic and Hepatic Macrophages and Peripheral Blood Mononuclear cells

Project description:Sickle cell disease and Beta-thalassemia represent hemoglobinopathies arising from dysfunctional or under produced beta-globin chains, respectively. In both diseases, red blood cell injury and anemia are the impetus for end organ injury. Because persistent erythrophagocytosis is a hallmark of these genetic maladies it is critical to understand how macrophage phenotype polarizations in tissue compartments can inform on disease progression. Murine models of sickle cell disease and Beta-thalassemia allow for a basic understanding of mechanisms and provide for translation to human disease. A multi-omics approach to understanding macrophage metabolism and protein changes in two murine models of beta-globinopathy was performed on peripheral blood mononuclear cells as well as spleen and liver macrophages isolated from Berkley sickle cell disease (Berk-ss) and heterozygous B1/B2 globin gene deletion (Hbbth3/+) mice. Results from these experiments revealed the metabolome and proteome of macrophages are polarized to a distinct phenotype in Berk-ss and Hbbth3/+ compared each other and their common background mice (C57BL6/J). Further, spleen and liver macrophages revealed distinct disease specific phenotypes, suggesting macrophages become differentially polarized and reprogrammed within tissue compartments. We conclude that tissue recruitment, polarization, metabolic and proteomic reprogramming of macrophages in Berk-ss and Hbb mice may be relevant to disease to progression in other tissue.

2023-07-17 | MSV000092457 | MassIVE

Immunophenotyping peripheral blood mononuclear cells in human HFpEF

Project description:Immunophenotyping peripheral blood mononuclear cells in human HFpEF

| PRJNA926645 | ENA

miR from peripheral blood mononuclear cells' (PBMCs') exosome

Project description:miR from peripheral blood mononuclear cells' (PBMCs') exosome

| PRJNA786954 | ENA