Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Ambystoma mexicanum strain:AGSC Lab Axolotl

ABSTRACT: Ambystoma mexicanum

PROVIDER: PRJNA33423 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR029144.fastq.gz	Fastqsanger.gz
	SRR029145.fastq.gz	Fastqsanger.gz
	SRR029146.fastq.gz	Fastqsanger.gz
	SRR029147.fastq.gz	Fastqsanger.gz
	SRR029148.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Ambystoma mexicanum x (Ambystoma mexicanum x Ambystoma tigrinum) breed:mexicanum/tigrinum hybrid

Project description:Ambystoma mexicanum x (Ambystoma mexicanum x Ambystoma tigrinum) breed:mexicanum/tigrinum hybrid Raw sequence reads

| PRJNA477812 | ENA

Ambystoma mexicanum

Project description:Ambystoma mexicanum regeneration map

| PRJNA300706 | ENA

Ambystoma mexicanum

Project description:Ambystoma mexicanum Raw sequence reads

| PRJNA306100 | ENA

Ambystoma mexicanum

Project description:Ambystoma mexicanum Raw sequence reads

| PRJNA480693 | ENA

Ambystoma mexicanum

Project description:Ambystoma mexicanum Transcriptome or Gene expression

| PRJNA291645 | ENA

Ambystoma mexicanum

Project description:Ambystoma mexicanum Genome sequencing and assembly

| PRJNA269757 | ENA

Tyrp1 is the mendelian determinant of the Axolotl (Ambystoma mexicanum) copper mutant

Project description:Several dozen Mendelian mutants have been discovered in axolotl (Ambystoma mexicanum) populations, including several that affect pigmentation. Four pigment mutants have been described in the scientific literature and genes for three of these have been identified. Here we report on copper, a Mendelian mutant with an albino-like phenotype known only from the pet trade. To identify the gene for copper, we performed a cross segregating copper and wildtype color phenotypes and used Bulked Segregant RNA-Seq to identify a region on chromosome 6 that was enriched for single-nucleotide polymorphisms (SNPs) between the color phenotypes. This region included Tyrosinase-like Protein 1 (Tyrp1), a melanin synthesis protein that when mutated, is associated with lighter than black melanin coloration in animal models and oculocutaneous albinism in humans. Inspection of RNA-Seq reads identified a single nucleotide deletion that is predicted to change the coding frame, introducing a premature stop codon in exon 6 and yield a truncated Tyrp1 protein in copper individuals. Using CRISPR-Cas9 editing, we show that wildtype Tyrp1 crispants exhibit copper pigmentation, thus confirming Tyrp1 as the copper locus. Our results suggest that commercial and hobbyist axolotl populations may harbor useful mutants for biological research.

2024-10-22 | GSE269079 | GEO

RNA_Seq analysis of localization along animal-vegetal axis of axolotl (Ambystoma mexicanum)

Project description:Asymmetrical localization of biomolecules inside the egg, results in uneven cell division and two daughter cells with different fates. This phenomenon is required for the establishment of many biological processes and is particularly responsible for the great variety of cell types formed during developmentand requires strict timing and positional control. The key molecules determining the body plan are the mRNAs, of which many examples have already been discovered to be asymmetrically localized during oogenesis and embryogenesis in both the amphibian and fish models. However, our knowledge about evolutionary conservation or differences of localized mRNAs is still limited to a few candidates. Our goal has been to compare localization profiles along the animal-vegetal axis of mature eggs of four diverse models, Xenopus laevis, Danio rerio, Ambystoma mexicanum and Acipenser ruthenus using the spatial expression analysis method called TOMO-Seq. Surprisingly, we revealed RNAs that code for many known important genes such as germ layer determinants, germ plasm factors and members of key signalling pathways, are localized in completely different profiles among the models and sometimes even missing in their genomes. We determined the transcriptome distribution and found a poor correlation between the vegetally localized genes but a relatively good correlation between the animally localized genes. These findings indicate that the regulation of embryonic development within the animal kingdom is highly diverse and cannot be deduced based on a single model.

2022-10-20 | GSE166916 | GEO

Ambystoma mexicanum strain:d/d

Project description:Ambystoma mexicanum brain regeneration time-course

| PRJNA520877 | ENA

Ambystoma mexicanum strain:DD151

Project description:Ambystoma mexicanum strain:DD151 Genome sequencing and assembly

| PRJNA378970 | ENA