Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Morus alba

ABSTRACT: Identification and Comparative Analysis of Drought Responsive microRNA Targets in Mulberry (Morus alba L.) by Degradome Sequencing

PROVIDER: PRJNA335564 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR3955481.fastq.gz	Fastqsanger.gz
	SRR3955482.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Transcriptome analysis of Indian mulberry leaf tissue

Project description:The mian goal of the study was to identify drought responsive genes of Indian mulberry (Morus alba L.) leaf tissue. Drought stress was imposed at whole plant leve; usig gravimetric approach. Leaf tissue was harvested 14 days post drought stress imposition and used for transcriptome analaysis. Two levels of drought stress (100% and 40% soil field capacity) was maintained by controlled irrigation. The leaf tissue was collected, total RNA was isolated converted to cDNA and used for analysis

2021-08-25 | GSE156822 | GEO

Identification and Comparative Analysis of Drought Responsive microRNA Targets in Mulberry (Morus alba L.) by Degradome Sequencing

Project description:MicroRNAs (miRNAs) are endogenous non-coding small RNAs containing 21-24 nucleotides, and play an important function in the course of stand up to adversity. Identification of miRNA target mRNAs is essential for their functional analysis. In order to anatomize miRNA guided gene regulation under drought stress, we performed a transcriptome-wide experimental method using high throughput degradome sequencing to directly detect drought stress responsive miRNA targets in mulberry. In this study, drought library (DL) and contrast library (CL) which captured the cleaved mRNA were constructed from mulberry for sequencing by Illumina sequencer, and 409, 373 target genes for 30 conserved miRNA families and 990, 950 target genes for 199, 195 novel miRNAs were identified in CL and DL, respectively. Among those conserved miRNA families in DL, mno-miR156, mno-miR172 and mno-miR396 refer to the highest number of targets, indicating that mno-miR156, mno-miR172 and mno-miR396 families and their target genes might play a key important function in corresponding to drought stress in mulberry. By construction and analysis of miRNA-target network, we found that the DL independent networks consist of 838 miRNA-mRNA pairs (63.34%). Besides, the expression patterns of 11 target genes and 12 correspondent miRNAs were compared and analyzed by qRT-PCR. In addition, 5 of 6 miRNA targets were further verified by RNA ligase-mediated 5’ rapid amplification of cDNA ends (RLM-5’ RACE). Gene ontology (GO) annotations and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis showed that these target transcripts were implicated in a broad range of biological processes and various metabolic pathways. The characterization of target genes and the associated miRNAs in response to drought exposure provides a framework for understanding the molecular mechanism of resistance to drought in plants.

2017-02-14 | GSE84889 | GEO

Morus alba

Project description:RNA-seq of mulberry (Morus alba L.)

| PRJNA597172 | ENA

Morus alba Raw protein sequence reads

Project description:Morus alba L. Raw protein sequence reads and sequence

2024-02-08 | PXD049451 |

Morus alba

Project description:Morus alba L sequencing

| PRJNA325563 | ENA

Gene expression analysis of mulberry (Morus atropurpurea) response to cadmium stress

Project description:Mulberry (Morus atropurpurea) is an important economic woody tree with rapid growth rate and large biomass, which had great potential for heavy metals remediation. To further understand the mechanisms involved in cadmium accumulation and detoxification in mulberry, we carried out a transcriptomic study to get insights into the molecular mechanisms of the mulberry response to cadmium stress using RNA-seq analysis with BGISEQ-500.

2022-06-30 | GSE152672 | GEO

Morus alba

Project description:Morus alba Raw sequence reads

| PRJNA1074415 | ENA

Morus alba

Project description:Morus alba Raw sequence reads

| PRJNA954546 | ENA

Morus alba

Project description:Morus alba Raw sequence reads

| PRJNA438058 | ENA

Morus alba

Project description:Morus alba Raw sequence reads