Escherichia coli REL606
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA336011 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Escherichia coli REL606 Raw sequence reads
| PRJNA385021 | ENA
Project description:Escherichia coli REL606 Raw RNA Sequence Reads
| PRJNA553503 | ENA
Project description:Escherichia coli REL606 Genome sequencing
| PRJNA294166 | ENA
Project description:Escherichia coli REL606 Genome sequencing
| PRJNA189495 | ENA
Project description:Investigating the evolution of Escherichia coli in microgravity offers valuable insights into microbial adaptation to extreme environments. Here the effects of simulated microgravity (SµG) on gene expression of E. coli REL606, a strain evolved terrestrially for 35 years is explored. We evaluated the transcriptomic changes for glucose-limited and glucose-replete conditions over 24 hours which illustrate that SµG increased the expression of stress response and cell membrane-related genes, particularly under glucose-limited conditions. A machine learning model predicted that glucose-limited SµG impacts the cellular membrane, while glucose-replete SµG also inhibits protein synthesis at stationary phase. These findings highlight the transcriptomic and physiological adaptations of E. coli to short term microgravity, offering a foundation for future research into the long-term effects of space conditions on bacterial evolution.
2025-05-13 | GSE284563 | GEO
Project description:The purpose of this study is to determine whether the presence of pathogenic Escherichia coli in colon is associated with psychiatric disorders.
| 2230352 | ecrin-mdr-crc
Project description:Study of the mechanisms of RecB mutant terminus DNA loss in Escherichia coli. FX158: WT MG1655 FX35: recB- FX37: ruvAB- FX51: matP- MIC18: recB- sbcD- sbcC- MIC20: recB- ruvAB- MIC24: matP- recB- MIC25: recA- recB- MIC31: sbcB- sbcD- MIC34: recA- recD- MIC40: linear chromosome MIC41: linear chromosome recB- MIC42: matP- ftsKC- MIC43: matP- ftsKC- recB- MIC48: recA- Cells were grown in M9 minimal medium supplemented with 0.4 % glucose to exponential phase (0.2 OD 650 nm). Chromosomal DNA was extracted using the Sigma GenElute bacterial genomic DNA kit. 5 μg of DNA were used to generate a genomic library according to Illumina's protocol. The libraries and the sequencing were performed by the High-throughput Sequencing facility of the I2BC (http://www.i2bc.paris-saclay.fr/spip.php?article399&lang=en, CNRS, Gif-sur-Yvette, France). Genomic DNA libraries were made with the ‘Nextera DNA library preparation kit’ (Illumina) following the manufacturer’s recommendations. Library quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit (Agilent technologies). Libraries were pooled in equimolar proportions. 75 bp single reads were generated on an Illumina MiSeq instrument, using a MiSeq Reagent kit V2 (500 cycles) (Illumina), with an expected depth of 217X. An in-lab written MATLAB-based script was used to perform marker frequency analysis. Reads were aligned on the Escherichia coli K12 MG1655 genome using BWA software. Data were normalized by dividing uniquely mapping sequence reads by the total number of reads. Enrichment of uniquely mapping sequence reads in 1 kb non-overlapping windows were calculated and plotted against the chromosomal coordinates.
2017-11-10 | E-MTAB-6122 | biostudies-arrayexpress
Project description:Despite the characterization of many aetiologic genetic changes. The specific causative factors in the development of sporadic colorectal cancer remain unclear. This study was performed to detect the possible role of Enteropathogenic Escherichia coli (EPEC) in developing colorectal carcinoma.
| 2184564 | ecrin-mdr-crc
Project description:Comparison of Escherichia coli proteomics of different DNA sequence binding proteins and identification of heterologous expressed protein
2021-04-14 | PXD025380 |