Marine metagenome
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA336648 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Marine microbial communities from Delaware Coast, sample from Delaware MO Early Summer May 2010 metatranscriptome
| PRJNA366581 | ENA
Project description:Marine microbial communities from Delaware Coast, sample from Delaware MO Early Summer May 2010 metagenome
| PRJNA336874 | ENA
Project description:Marine microbial communities from Delaware Coast, sample from Delaware MO Late spring/early summer metatranscriptome
| PRJNA366580 | ENA
Project description:Marine microbial communities from Delaware Coast, sample from Delaware MO Summer July 2011 metagenome
| PRJNA336828 | ENA
Project description:Marine microbial communities from Delaware Coast, sample from Delaware MO Winter December 2010 metatranscriptome
| PRJNA366579 | ENA
Project description:Marine microbial communities from Delaware Coast, sample from Delaware MO Spring March 2010 metagenome
| PRJNA336752 | ENA
Project description:Marine microbial communities from Delaware Coast, sample from Delaware MO Winter December 2010 metagenome
| PRJNA336873 | ENA
Project description:The marine diatom Guinardia delicatula is a cosmopolitan species that dominates seasonal blooms in the English Channel and the North Sea. Several eukaryotic parasites are known to induce the mortality of this key-stone species. Here, we report the isolation and the characterization of the first viruses that infect G. delicatula. Viruses were isolated from the Western English Channel (SOMLIT-ASTAN station) during the late summer bloom decline of G. delicatula. A combination of laboratory approaches revealed that these lytic viruses (GdelRNAV) are small untailed particles of 35-38 nm in diameter that replicated in the host cytoplasm where both unordered particles and crystalline arrays were formed. GdelRNAV displayed a linear single-stranded RNA genome of ~9 kb, including two open reading frames encoding for replication and structural polyproteins. Phylogenetic relationships based on the RNA-dependent-RNA-polymerase gene marker showed that GdelRNAV were new members of the Bacillarnavirus, a monophyletic genus belonging to the order Picornavirales. GdelRNAV were specific to several strains of G. delicatula, they were produced rapidly (< 12h) and in numbers (9.34 x 104 virions per host cell). We recorded a substantial delay (72 h) between virions release and host cell lysis. Our analysis points to variable viral susceptibilities of the host during the early exponential growth phase. Interestingly, we consistently failed to isolate viruses during spring and early summer while G. delicatula developed rapid and massive blooms. While our study suggests that viruses do contribute to the decline of G. delicatula late summer bloom, they may not be the primary mortality agents during the remaining blooms at SOMLIT-ASTAN. Future studies should focus on the relative contribution of the viral and eukaryotic pathogens to the control of Guinardia blooms to understand the fate of these prominent organisms in marine systems.
2019-01-30 | PXD010967 | Pride
Project description:Purpose: To study small RNAs expressed in A. thaliana flowers Methods: sRNA libraries were constructed as previously described (C Lu et al. 2007) and sequenced on an Illumina GAIIx or Illumina HiSeq 2000 instrument at the Delaware Biotechnology Institute. These libraries were prepared between 2010 and 2012.
2018-08-01 | GSE97917 | GEO
Project description:To characterize the taxonomic and functional diversity of biofilms on plastics in marine environments, plastic pellets (PE and PS, ø 3mm) and wooden pellets (as organic control) were incubated at three stations: at the Baltic Sea coast in Heiligendamm (coast), in a dead branch of the river Warnow in Warnemünde (inlet), and in the Warnow estuary (estuary). After two weeks of incubation, all pellets were frozen for subsequent metagenome sequencing and metaproteomic analysis. Biofilm communities in the samples were compared on multiple levels: a) between the two plastic materials, b) between the individual incubation sites, and c) between the plastic materials and the wooden control. Using a semiquantitative approach, we established metaproteome profiles, which reflect the dominant taxonomic groups as well as abundant metabolic functions in the respective samples.
2021-11-01 | PXD012062 | Pride