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Soil metagenome

ABSTRACT: Amended soil microbial communities from Kansas Great Prairies, USA - Total DNA F1.4 TB amended with BrdU and acetate no abondance metagenome

PROVIDER: PRJNA336868 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4029452_1.fastq.gz	Fastqsanger.gz
	SRR4029452_2.fastq.gz	Fastqsanger.gz
	SRR4029453_1.fastq.gz	Fastqsanger.gz
	SRR4029453_2.fastq.gz	Fastqsanger.gz

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soil metagenome

Project description:Amended soil microbial communities from Kansas Great Prairies, USA - acetate Total DNA F2.4TB metagenome

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Analysis of the physiological status of Geobacter uraniireducens growing in uranium-contaminated subsurface sediments

Project description:Geobacteraceae transfer electrons from a donor such as acetate to an electron acceptor such as Fe(III) or U(VI). Geobacter uraniireducens is found in uranium-contaminated sites and plays an important role in in situ bioremediation. In this experiment, gene expression was compared between G. uraniireducens cultures grown in sediments from a uranium contaminated site amended with acetate and cultures grown in acetate/fumarate medium. Keywords: two-condition comparison

2008-10-20 | GSE10920 | GEO

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Cadmium specific proteomic responses of Pseudomonas aeruginosa san ai

Project description:Pseudomonas aeruginosa san ai resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to sub-lethal concentration of cadmium (0.9 mM) adsorbed 75% of added cadmium after 48 hours, while remaining 25% in culture supernatant was adsorbed by exopollysaccaryde, implying a large biosorption potential of leaving biomass and its exopollysaccaryde. Inner cell response on the protein level was investigated by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins in complex protein fractions obtained by size exclusion chromatography used to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 59 proteins were observed as up-regulated in cadmium- amended culture. Almost a third of the total number of up-regulated were metalloproteins. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in cell energy metabolism, amino acid metabolism, cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium- amended culture versus control was found. Our results signify that P. aeruginosa san ai has a great potential for application in bioremediation of cadmium polluted sites.

2022-05-19 | PXD008020 | Pride

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