Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Escherichia coli strain:LF82

ABSTRACT: Adherent-invasive strain

PROVIDER: PRJNA33825 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	GCA_000284495.1_sequence_report.txt	Txt

Items per page:

1 - 1 of 1

Similar Datasets

Escherichia coli LF82

Project description:Adherent-Invasive Escherichia coli Transcriptome

| PRJNA752090 | ENA

Escherichia coli LF82

Project description:Adherent-Invasive E. Coli (AIEC) strains are immune to most antibiotics unless two different antibiotics are used in succession

| PRJNA487828 | ENA

Escherichia coli

Project description:Comparative Genomics of Adherent Invasive E. coli

| PRJNA1074774 | ENA

Inflammatory bowel disease-associated <i>Escherichia coli</i> strain LF82 in the damage of gut and cognition of honeybees.

Project description:Patients with inflammatory bowel disease (IBD) are often accompanied with some cognitive impairment, but the mechanism is unclear. By orally exposing honeybees (Apis mellifera) to IBD-associated Escherichia coli LF82 (LF82), and non-pathogenic Escherichia coli MG1655 (MG1655) as the normal strain, we investigated whether and how LF82 induces enteritis-like manifestations and cognitive behavioral modifications in honeybees using multiparametric analysis. LF82 significantly increased gut permeability, impaired learning and memory ability in olfactory proboscis extension response conditioning, and shortened the lifespan of honeybees. Compared to MG1655, LF82 reduced the levels of tryptophan metabolism pathway substances in the honeybee gut. LF82 also upregulated genes involved in immune and apoptosis-related pathways and downregulated genes involved in G protein-coupled receptors in the honeybee brain. In conclusion, LF82 can induce enteritis-like manifestations and cognition impairment through gut metabolites and brain transcriptome alteration in honeybees. Honeybees can serve as a novel potential model to study the microbiota-gut-brain interaction in IBD condition.

| S-EPMC9453226 | biostudies-literature

Complete genome sequence of Crohn's disease-associated adherent-invasive E. coli strain LF82.

Project description:BACKGROUND: Ileal lesions of Crohn's disease (CD) patients are abnormally colonized by pathogenic adherent-invasive Escherichia coli (AIEC) able to invade and to replicate within intestinal epithelial cells and macrophages. PRINCIPAL FINDINGS: We report here the complete genome sequence of E. coli LF82, the reference strain of adherent-invasive E. coli associated with ileal Crohn's disease. The LF82 genome of 4,881,487 bp total size contains a circular chromosome with a size of 4,773,108 bp and a plasmid of 108,379 bp. The analysis of predicted coding sequences (CDSs) within the LF82 flexible genome indicated that this genome is close to the avian pathogenic strain APEC_01, meningitis-associated strain S88 and urinary-isolated strain UTI89 with regards to flexible genome and single nucleotide polymorphisms in various virulence factors. Interestingly, we observed that strains LF82 and UTI89 adhered at a similar level to Intestine-407 cells and that like LF82, APEC_01 and UTI89 were highly invasive. However, A1EC strain LF82 had an intermediate killer phenotype compared to APEC-01 and UTI89 and the LF82 genome does not harbour most of specific virulence genes from ExPEC. LF82 genome has evolved from those of ExPEC B2 strains by the acquisition of Salmonella and Yersinia isolated or clustered genes or CDSs located on pLF82 plasmid and at various loci on the chromosome. CONCLUSION: LF82 genome analysis indicated that a number of genes, gene clusters and pathoadaptative mutations which have been acquired may play a role in virulence of AIEC strain LF82.

| S-EPMC2941450 | biostudies-literature

Transcriptional profile of AIC Escherichia coli LF82 wild-type vs their delta-nrdR mutant.

Project description:We aim to compare global transcriptomic analysis of wt and delta-nrdRmutant in Escherichia coli LF82 strain.

2015-02-02 | E-MTAB-2288 | biostudies-arrayexpress

Transcriptional profile of AIC Escherichia coli LF82 wild-type vs their delta nrdR mutant.

Project description:We aim to compare global transcriptomic analysis of wt and delta nrdRmutant in Escherichia coli LF82 strain.

2016-06-27 | E-MTAB-2295 | biostudies-arrayexpress

The Crohn's disease-associated Escherichia coli strain LF82 relies on SOS and stringent responses to survive, multiply and tolerate antibiotics within macrophages.

Project description:Adherent Invasive Escherichia coli (AIEC) strains recovered from Crohn's disease lesions survive and multiply within macrophages. A reference strain for this pathovar, AIEC LF82, forms microcolonies within phagolysosomes, an environment that prevents commensal E. coli multiplication. Little is known about the LF82 intracellular growth status, and signals leading to macrophage intra-vacuolar multiplication. We used single-cell analysis, genetic dissection and mathematical models to monitor the growth status and cell cycle regulation of intracellular LF82. We found that within macrophages, bacteria may replicate or undergo non-growing phenotypic switches. This switch results from stringent response firing immediately after uptake by macrophages or at later stages, following genotoxic damage and SOS induction during intracellular replication. Importantly, non-growers resist treatment with various antibiotics. Thus, intracellular challenges induce AIEC LF82 phenotypic heterogeneity and non-growing bacteria that could provide a reservoir for antibiotic-tolerant bacteria responsible for relapsing infections.

| S-EPMC6855411 | biostudies-literature

Escherichia coli LF82

Project description:Escherichia coli LF82 Transcriptome or Gene expression

| PRJNA1070047 | ENA

Escherichia coli LF82

Project description:Gut colonization of AIEC

| PRJDB10678 | ENA