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Mus musculus

ABSTRACT: Single cell transcriptome sequencing of mammary stem cells in the pubertal mammary gland

PROVIDER: PRJNA339617 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4045660_1.fastq.gz	Fastqsanger.gz
	SRR4045660_2.fastq.gz	Fastqsanger.gz
	SRR4045661_1.fastq.gz	Fastqsanger.gz
	SRR4045661_2.fastq.gz	Fastqsanger.gz
	SRR4045662_1.fastq.gz	Fastqsanger.gz

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Single cell transcriptome sequencing of mammary stem cells in the pubertal mammary gland

Project description:The mammary gland is a highly dynamic organ that mainly develops during puberty. Based on morphology and proliferation analysis, mammary stem cells (MaSCs) are thought to be close to or reside in the terminal end buds (TEBs) during pubertal development. However, exclusive stem cell markers are lacking, and therefore the true identity of MaSCs, including their location, multiplicity, dynamics and fate during branching morphogenesis, has yet to be defined. To gain more insights into the molecular identity and heterogeneity of the MaSC pool, we performed single cell transcriptome sequencing of mammary epithelial cells micro-dissected from ducts and TEBs during puberty. These data show that the behaviour of MaSCs cannot be directly linked to a single expression profile. Instead, morphogenesis of the mammary epithelium relies upon a heterogeneous population of MaSCs that functions long-term as a single equipotent pool of stem cells.

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Mus musculus hematopoietic stem cell

Project description:A transcriptome study in mouse hematopoietic stem cells was performed using a sensitive SAGE method, in an attempt to detect medium and low abundant transcripts expressed in these cells. Among a total of 31,380 unique transcript, 17,326 (55%) known genes were detected, 14,054 (45%) low-copy transcripts that have no matches to currently known genes. 3,899 (23%) were alternatively spliced transcripts of the known genes and 3,754 (22%) represent anti-sense transcripts from known genes.

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RNA-Seq analysis of pubertal mammary epithelial cells reveals novel n-3 polyunsaturated fatty acid transcriptomic changes in the fat-1 mouse model

Project description:Background/Objectives: Early exposure of nutrients during pubertal mammary gland development may reduce the risk of developing breast cancer later in life. Anticancer, n-3 polyunsaturated fatty acids (n-3 PUFA) are shown to modulate pubertal mammary gland development, however, the mechanisms of action remains unclear. Prior work focuses on effects at the whole tissue level, and little is known at the cellular level, such as mammary epithelial cells (MEC), which are implicated in cancer development. Methods: This pilot study examined the effects of lifelong n-3 PUFA exposure on the transcriptome by RNA-Seq in isolated MEC of pubertal (6–8-week-old) female fat-1 transgenic mice capable of de novo n-3 PUFA synthesis. edgeR and DESeq2 were used separately for the differential expression analysis of RNA sequencing data followed by Benjamini-Hochberg procedure for multiple testing correction. Results: Nine genes were found concordant and significantly different (p ≤ 0.05) by both DESeq2 and edgeR methods. These genes were associated with multiple pathways, suggesting that n-3 PUFA stimulates estrogen-related signalling (Mllt10, Galr3, Nrip1) and a glycolytic profile (Soga1, Pdpr, Uso1) while offering protective effects for immune and DNA damage responses (Glpd1, Garre1, Rpa1) in MEC during puberty. Conclusions: This pilot study highlights the utility of RNA-Seq to better understand mechanistic effects of specific nutrients such as n-3 PUFA in a cell-specific manner. Thus, further studies are warranted to investigate cell-specific mechanisms by which n-3 PUFA influences pubertal mammary gland development and breast cancer risk later in life.

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SHARPIN regulates collagen architecture and ductal invasion in the developing mouse mammary gland

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Gene expression of RonTK-/- mammary glands compared to RonTK+/+ controls during development

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2009-06-16 | E-GEOD-16629 | biostudies-arrayexpress

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