Project description:The Chinese sturgeon (Acipenser sinensis) is anadromous fish distributed in Yangtze River and East China Sea. In this study, we reported cleft-palate Chinese sturgeons in artificial population for the first time. In order to explore the genetic basis of palate malformation in A. sinensis, Illumina RNA-seq technology was used to analyze the transcriptome data of normal and cleft-palate individuals in farmed Chinese sturgeons. Raw reads were obtained and assembled into 808,612 unigenes, with an average length of 509.33 bp and an N50 of 574 bp. Sequence similarity analyses against four public databases (Nr, Uniprot, KEGG and COGs) found 158,642 unigenes that can be annotated. GABAergic synapse and TGF-β signal pathway were the most two enriched pathways with high Richfactor in the analyses of different expressed genes. In these two signal pathways, six genes (GABRA4, GS, GNS, S6K, PITX2, and BMP8) were found as cleft-palate genes in Chinese sturgeon. These findings contribute to our understanding of the genetic basis of cleft palate in sturgeon, while simultaneously adding to our knowledge about craniofacial development.
Project description:Hybrid sturgeon, an ancient fish, has great economic and nutritional value as a source of caviar. Although significant differences exist in the female reproductive capacity under artificial breeding conditions, differential gene expression associated to domesticated female reproductive capacity have never been investigated. In this study, we conducted reference genome-based transcriptome sequencing of 16 individuals from high-yield (H) and low-yield (L) populations, respectively.
Project description:On October 9th,2023, one thousand Amur sturgeon with similar genetic background were released to Songhua River after artificial breeding in Harbin. Before re-wilding to river,they were bred in the net cages. To explore whether feeding Limnodrilus before stock enhancement would affect the gene expression mode, we set two groups, one group was fed with commerial feed , another was fed with fresh Limnodrilus. After 30 d breeding, we collected the gut samples to perform transcriptome sequencing project.