Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of a core p53 transcriptional program with highly fractionated tumor suppressive activity

ABSTRACT: Identification of a core p53 transcriptional program with highly fractionated tumor suppressive activity

PROVIDER: PRJNA340955 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR4090090.fastq.gz	Fastqsanger.gz
	SRR4090100.fastq.gz	Fastqsanger.gz
	SRR4098380.fastq.gz	Fastqsanger.gz
	SRR4098390.fastq.gz	Fastqsanger.gz
	SRR4098400.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 61

Similar Datasets

Identification of a core p53 transcriptional program with highly fractionated tumor suppressive activity

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-08-22 | GSE86222 | GEO

2019PREs_Particle-fractionated

Project description:2019PREs_Particle-fractionated Raw sequence reads

| PRJNA1084175 | ENA

Identification of a core TP53 transcriptional program with highly distributed tumor suppressive activity.

Project description:The tumor suppressor TP53 is the most frequently mutated gene product in human cancer. Close to half of all solid tumors carry inactivating mutations in the TP53 gene, while in the remaining cases, TP53 activity is abrogated by other oncogenic events, such as hyperactivation of its endogenous repressors MDM2 or MDM4. Despite identification of hundreds of genes regulated by this transcription factor, it remains unclear which direct target genes and downstream pathways are essential for the tumor suppressive function of TP53. We set out to address this problem by generating multiple genomic data sets for three different cancer cell lines, allowing the identification of distinct sets of TP53-regulated genes, from early transcriptional targets through to late targets controlled at the translational level. We found that although TP53 elicits vastly divergent signaling cascades across cell lines, it directly activates a core transcriptional program of ∼100 genes with diverse biological functions, regardless of cell type or cellular response to TP53 activation. This core program is associated with high-occupancy TP53 enhancers, high levels of paused RNA polymerases, and accessible chromatin. Interestingly, two different shRNA screens failed to identify a single TP53 target gene required for the anti-proliferative effects of TP53 during pharmacological activation in vitro. Furthermore, bioinformatics analysis of thousands of cancer genomes revealed that none of these core target genes are frequently inactivated in tumors expressing wild-type TP53. These results support the hypothesis that TP53 activates a genetically robust transcriptional program with highly distributed tumor suppressive functions acting in diverse cellular contexts.

| S-EPMC5630028 | biostudies-literature

MCF-7 Fractionated RNA

Project description:RNA from MCF-7 cells was fractionated by sucrose density gradient centrifugation to separate RNA associated with membrane-bound polysomes from RNA associated with free polysomes. These two populations were hybridized in triplicate to U133A microarrays. Keywords: repeat sample

2004-12-01 | GSE1400 | GEO

p53 dimers elicit unique tumor suppressive activities through an altered metabolic program

Project description:p53 dimers elicit unique tumor suppressive activities through an altered metabolic program

| PRJNA912177 | ENA

Next Generation Sequencing of total RNA from 9 bladder cancer cell lines and 3 fractionated bladder cancer cell lines

Project description:We profile the cell line expression of 279 circRNAs, that are highly expressed across 457 bladder cancer patient samples. Additionally, we investigate their cellular location in fractionated cell lines

2017-11-01 | GSE100971 | GEO

Transcriptional correlates of FOXP3 expression in suppressive and non-suppressive primary human Treg cell clones.

Project description:Analysis of the transcriptional correlates of FOXP3 expression in suppressive and non-suppressive primary human Treg cell clones. Individual CD4+CD25High or Cd4+CD25- T cells were isolated from human PBMCs and expanded in vitro. After 3 weeks of expansion, individual clones were analysed for FOXP3 expression and in vitro suppressive activity against freshly sorted allogeneic effector T cells. This study analyses the total RNA isolated from FOXP3+ clones with suppressive potency to their non-suppressive counterparts. The resutls of this study should provide insights into the molecular pathways linking FOXP3 expression to distinct aspects of Treg phenotype and function. Total RNA obtained from individual clones of primary human regulatory and effector CD4+T cells.

2015-05-06 | E-GEOD-65650 | biostudies-arrayexpress

p53 dimers elicit unique tumor suppressive activities through an altered metabolic program [WES]

Project description:p53 dimers elicit unique tumor suppressive activities through an altered metabolic program [WES]

| PRJNA912181 | ENA

Dissemination of size-fractionated antibiotic resistance genes

Project description:Dissemination of size-fractionated antibiotic resistance genes

| PRJEB46883 | ENA

p53 dimers elicit unique tumor suppressive activities through an altered metabolic program [RNA-seq]

Project description:p53 dimers elicit unique tumor suppressive activities through an altered metabolic program [RNA-seq]

| PRJNA912180 | ENA