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Rattus norvegicus

ABSTRACT: TonEBP/NFAT5 regulates inflammatory gene expression in nucleus pulposus cells

PROVIDER: PRJNA342179 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4192020.fastq.gz	Fastqsanger.gz
	SRR4192021.fastq.gz	Fastqsanger.gz
	SRR4192022.fastq.gz	Fastqsanger.gz
	SRR4192015.fastq.gz	Fastqsanger.gz
	SRR4192016.fastq.gz	Fastqsanger.gz

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Rattus norvegicus

Project description:TonEBP/NFAT5 controls inflammatory transcriptional response to TNF-a in nucleus pulposus cells

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TonEBP/NFAT5 regulates inflammatory gene expression in nucleus pulposus cells

Project description:RNA sequencing of nucleus pulposus cells transduced with control or TonEBP shRNA and cultured under hypertonic conditions

2016-11-08 | GSE86552 | GEO

Effect of NFAT5 deficiency on gene expression in BV2 cells

Project description:Tonicity-Responsive Enhancer-Binding Protein (NFAT5), also known as Tonicity-Responsive Enhancer Binding Protein (TonEBP), is a important transcription factor in the regulation of osmoprotective and inflammatory genes. NFAT5 has been reported to regulate the pathological processes of inflammatory and autoimmune disorders. Our data provides a insight to the gene expression in NFAT5 deficiency BV2 microglia cells, which NFAT5 knocked down by sh-RNA.

2024-02-15 | GSE249968 | GEO

TonEBP/NFAT5 controls inflammatory transcriptional response to TNF-a in nucleus pulposus cells

Project description:RNA sequencing of nucleus pulposus cells transduced with shRNA (control or TonEBP-targeted) and either untreated or treated with TNF-a (24h)

2017-08-24 | GSE97496 | GEO

Rattus norvegicus

Project description:rat nucleus pulposus transcriptome sequencing

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TonEBP-deficiency accelerates age-dependent intervertebral disc degeneration underscored by matrix remodeling, cytoskeletal rearrangements, and changes in proinflammatory gene expression

Project description:Our studies show that TonEBP-deficiency causes pronounced degeneration of all three intervertebral disc compartments with greater incidence of herniation in the mouse. The disc phenotype is marked by extracellular matrix remodeling, actin cytoskeleton rearrangements, and suppressed proinflammatory gene expression, advancing our understanding of the contributions of TonEBP in intervertebral disc homeostasis and disease. We used microarray to explore the transcriptomics of differentially expressed genes of annulus fibrosus (AF) and nucleus pulposus (NP) tissue in TonEBP haploinsufficient mice on a C57BL/6 background.

2021-08-05 | GSE135270 | GEO

Expression data from synovium, bone marrow and nucleus pulposus

Project description:Synovial and bone marrow mesenchymal stem cells after intradiscally injection show regenerative effects of nucleus pulposus. Microarray analyses of rats were performed to investigate the closeness of the gene profiles between the nucleus pulposus cells and the synovial or bone marrow mesenchymal stem cells. To investigate interaction between synovial mesenchymal stem cells and nucleus pulposus cells, human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured, and species specific microarray were performed. Synovium of knee joints, bone marrow and nucleus pulposus were harvested from rat or human, and cells were isolated for RNA extraction and hybridization on Affymetrix microarrays. To compare the gene profiles each other, isolated cells were mono-cultured respectively, and human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured.

2010-10-11 | E-GEOD-24612 | biostudies-arrayexpress

Impact of Nfat5 on normoxia- and hypoxia-exposed mouse lung endothelial cells

Project description:Chronic hypoxia causes detrimental structural alterations in the lung, which are partially dependent on stress responses of the endothelium. In this context, we revealed that hypoxia-exposed murine lung endothelial cells (MLEC) activate nuclear factor of activated T-cells 5 (NFAT5/TonEBP) - a transcription factor that adjusts the cellular transcriptome to cope with multiple environmental stressors. Here, we studied the impact of NFAT5 on hypoxia-induced gene expression in MLEC by comparing the transcriptome of control and Nfat5-deficient MLEC, which were isolated from mouse lungs after exposure to normoxia and hypoxia for 7 days and processed for scRNA seq.

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Analysis of transcriptome in normoxia/hypoxia(21d)-exposed lungs upon KO of Nfat5 in ECs

Project description:Chronic hypoxic stress stimulates lung endothelial cells to promote vascular remodeling processes, which - in the long run - increase the resistance of pulmonary arteries. While several molecular determinants promoting these maladaptive changes have been delineated, their transcriptional regulation is not well studied. In this context, we revealed that hypoxia activates nuclear factor of activated T-cells 5 (NFAT5/TonEBP) in murine lung endothelial cells (MLECs) - a transcription factor that regulates the adjustment of the cellular transcriptome to cope with osmotic, biomechanical or metabolic environmental stressors. Here, we studied the functional relevance of NFAT5 for the control of endothelial hypoxic stress responses in the lung. Genetic ablation of Nfat5 in endothelial cells did not evoke any obvious phenotypic alterations under normoxia. However, microarray-based transcriptome analyses of lung tissue revealed significant alterations 7 but not 21 days after exposure to normobaric hypoxia (10% O2).

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