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Homo sapiens

ABSTRACT: An ultra effective method of generating extra multipotent cells from human fibroblasts via ultrasound-directed instant chromatin remodeling [MBD-seq]

PROVIDER: PRJNA342477 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4217782_1.fastq.gz	Fastqsanger.gz
	SRR4217782_2.fastq.gz	Fastqsanger.gz
	SRR4217783_1.fastq.gz	Fastqsanger.gz
	SRR4217783_2.fastq.gz	Fastqsanger.gz
	SRR4217784_1.fastq.gz	Fastqsanger.gz

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An ultra effective method of generating extra multipotent cells from human fibroblasts via ultrasound-directed instant chromatin remodeling [MBD-seq]

Project description:We have developed an ultra effective method of generating extra multipotent (xpotent) cells which have differentiation potential more than limited cell types, by the mechanism of ultrasound-directed permeation of environmental transition-guided cellular reprogramming (Entr).

2019-09-09 | GSE86647 | GEO

An ultra effective method of generating extra multipotent cells from human fibroblasts via ultrasound-directed instant chromatin remodeling

2019-09-09 | GSE86646 | GEO

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Project description:An ultra effective method of generating extra multipotent cells from human fibroblasts via ultrasound-directed instant chromatin remodeling

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Ultrasound-mediated ultra effective cellular reprogramming of human fibroblasts into neural progenitor-like cells [MBD-Seq]

Project description:We have developed an ultra effective method of triggering fast cellular reprogramming into neural progenitor cells (NPCs) with significant yields, called ultrasound-directed permeation of environmental transition-guided cellular reprogramming (Entr).

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Ultrasound-mediated ultra effective cellular reprogramming of human fibroblasts into neural progenitor-like cells [RNA-Seq]

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Project description:Ultrasound-mediated ultra effective cellular reprogramming of human fibroblasts into neural progenitor-like cells [MBD-Seq]

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Cell-free DNA methylome profiling by MBD-seq with ultra-low input

Project description:Here, we report an enrichment-based ultra-low input cfDNA methylation profiling method using methyl-CpG binding proteins capture, termed cfMBD-seq. We optimized the conditions of cfMBD capture by adjusting the amount of MethylCap protein along with using methylated filler DNA. Our data showed that cfMBD-seq performs equally to the standard MBD-seq (>1000 ng input) even when using 1 ng DNA as the input. cfMBD-seq demonstrated equivalent sequencing data quality as well as similar methylation profile when compared to cfMeDIP-seq. We showed that cfMBD-seq outperforms cfMeDIP-seq in the enrichment of CpG islands. This new bisulfite-free ultra-low input methylation profiling technology has a great potential in non-invasive and cost-effective cancer detection and classification.

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Ultrasound-mediated ultra effective cellular reprogramming of human fibroblasts into neural progenitor-like cells

Project description:Ultrasound-mediated ultra effective cellular reprogramming of human fibroblasts into neural progenitor-like cells

| PRJNA343878 | ENA

Homo sapiens

Project description:Ultrasound-mediated ultra effective cellular reprogramming of human fibroblasts into neural progenitor-like cells [RNA-Seq]

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Quality evaluation of Methyl Binding Domain based kits for enrichment DNA-methylation sequencing

Project description:Purpose: to compare different Methyl Binding Domain (MBD) based kits for DNA-methylation sequencing using Reduced Representation Bisulfite Sequencing (RRBS) data for validation, and to determine whether data quality can also be derived from inherent sequence data characteristics MBD-seq using 5 different kits (MethylCap, MethylCollector, MethylCollector Ultra, MethylMiner, MethylMagnet) was applied on 3 commonly used cell lines (DU145, HCT15, PC3), for which also RRBS data were generated.

2013-01-23 | E-GEOD-42790 | biostudies-arrayexpress

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