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Streptomyces ambofaciens ATCC 23877

ABSTRACT: Streptomyces ambofaciens time resolved transcriptome

PROVIDER: PRJNA342588 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4238011.fastq.gz	Fastqsanger.gz
	SRR4238002.fastq.gz	Fastqsanger.gz
	SRR4238012.fastq.gz	Fastqsanger.gz
	SRR4237983.fastq.gz	Fastqsanger.gz
	SRR4238013.fastq.gz	Fastqsanger.gz

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Prophage induction can facilitate the in vitro dispersal of multicellular Streptomyces structures - Transcriptome of Streptomyces ambofaciens ATCC 23877 grown 30 h on solid media (HT, SAF, ONA, MMM, MMM+NAG)

Project description:The terminal compartments of Streptomyces are less prone to transcription than the rest of the chromosome. Indeed, the expression of the highly variable regions enriched in those compartments is generally conditional and often requires an empirical approach to characterize the inducing conditions. For instance, in the context of identifying adequate antibiotic production conditions, an OSMAC (“One Strain Many Compounds”) approach is frequently implemented, based on strain cultivation in different environmental conditions (composition of the medium, growth time, temperature, co-cultures, etc.). Likewise, to find the expression conditions of a complete prophage of Streptomyces ambofaciens ATCC 23877 (named 'Samy' phage/prophage), we conducted a similar approach by analyzing the transcriptomes in five solid media (HT, SAF, ONA, MMM, MMM+NAG). The terminal compartments of Streptomyces are less prone to transcription than the rest of the chromosome. Indeed, the expression of the highly variable regions enriched in those compartments is generally conditional and often requires an empirical approach to characterize the inducing conditions. For instance, in the context of identifying adequate antibiotic production conditions, an OSMAC (“One Strain Many Compounds”) approach is frequently implemented, based on strain cultivation in different environmental conditions (composition of the medium, growth time, temperature, co-cultures, etc.). Likewise, to find the expression conditions of a complete prophage of Streptomyces ambofaciens ATCC 23877 (named 'Samy' phage/prophage), we conducted a similar approach by analyzing the transcriptomes in five solid media (HT, SAF, ONA, MMM, MMM+NAG).

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