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Aptenodytes patagonicus

ABSTRACT: Population genomics of king penguins

PROVIDER: PRJNA343012 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4244480_1.fastq.gz	Fastqsanger.gz
	SRR4244480_2.fastq.gz	Fastqsanger.gz
	SRR4244490_1.fastq.gz	Fastqsanger.gz
	SRR4244490_2.fastq.gz	Fastqsanger.gz
	SRR4244500_1.fastq.gz	Fastqsanger.gz

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Gene expression profile in the pectoralis muscle of penguin before and after acclimatisation to marine life

Project description:In the present study, we were interested in gene expression changes in the pectoralis muscle of juvenile king penguins during the transition from terrestrial to marine life. Strictly terrestrial during their first year after hatching, king penguin chicks must then depart to sea to reach nutritional emancipation and pectoralis muscle is largely involved in penguin adaptation to the marine environment. To compare these transcriptomic profiles, we realized heterologous hybridization on Affymetrix GeneChip Chicken Genome Arrays, as the chicken is the closest model species for which microarrays are available. The development of a new algorithm, MaxRS, allow us to determine differentially expressed genes implicated in energetic metabolism or involved in cellular defense against reactive oxygen species and associated injuries.

2010-05-25 | GSE17725 | GEO

Gene expression profile in the pectoralis muscle of penguin juveniles artificially acclimated to marine life

2016-08-31 | GSE82344 | GEO

Gene expression profile in the pectoralis muscle of penguin before and after acclimatisation to marine life

2010-06-08 | E-GEOD-17725 | biostudies-arrayexpress

Transcriptomic data analysis and differential gene expression of antioxidant pathways in king penguin juveniles (<i>Aptenodytes patagonicus</i>) before and after acclimatization to marine life.

Project description:In this article, we present differentially expressed gene profiles in the pectoralis muscle of wild juvenile king penguins that were either naturally acclimated to cold marine environment or experimentally immersed in cold water as compared with penguin juveniles that never experienced cold water immersion. Transcriptomic data were obtained by hybridizing penguins total cDNA on Affymetrix GeneChip Chicken Genome arrays and analyzed using maxRS algorithm, "Transcriptome analysis in non-model species: a new method for the analysis of heterologous hybridization on microarrays" (Dégletagne et al., 2010) [1]. We focused on genes involved in multiple antioxidant pathways. For better clarity, these differentially expressed genes were clustered into six functional groups according to their role in controlling redox homeostasis. The data are related to a comprehensive research study on the ontogeny of antioxidant functions in king penguins, "Hormetic response triggers multifaceted anti-oxidant strategies in immature king penguins (Aptenodytes patagonicus)" (Rey et al., 2016) [2]. The raw microarray dataset supporting the present analyses has been deposited at the Gene Expression Omnibus (GEO) repository under accessions GEO: GSE17725 and GEO: GSE82344.

| S-EPMC5061121 | biostudies-literature

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