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Effect of CCAR2 depletion on the gene expression profile of BJ-hTERT and U2OS cells

ABSTRACT: Effect of CCAR2 depletion on the gene expression profile of BJ-hTERT and U2OS cells

PROVIDER: PRJNA344492 | ENA |

REPOSITORIES: ENA

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Effect of CCAR2 depletion on the gene expression profile of BJ-hTERT cells

Project description:CCAR2 is a nuclear protein recently emerged as a pivotal player of the DNA damage response since it has been found involved in both apoptosis induction and DNA repair. Differently, its role in tumorigenesis and cancer progression is still elusive. In our studies we found that CCAR2 depletion impairs the proliferation of human cancer cell lines, but leaves unaffected the growth of normal immortalized cells. To better investigate this point we performed a genome wide gene expression analyses in U2OS and BJ-hTERT depleted of CCAR2 and we found that loss of this protein causes the deregulation of genes implicated in the AKT pathway specifically in U2OS cells, but not in BJ-hTERT. In accordance with these results we found a reduction in AKT activation in all the tested cancer cell lines depleted of CCAR2, but not in the normal ones. The defective activation of AKT is caused by the upregulation of TRB3 gene in cancer cells depleted of CCAR2 and finally results in the reduction of GSK3β phosphorylation, prevention of G1/S transition and inhibition of cancer cell growth.

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Effect of CCAR2 depletion on the gene expression profile of U2OS cells

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Transcription profiling of human BJ fibroblasts after LIN-9 depletion by a shRNA

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miRNA Expression in Senescent and Immortalized BJ fibroblasts

Project description:Limited data exists regarding changes of microRNA (miRNA) expression during senescence in human cells and no reports correlate telomerase expression with regulation of senescence-related miRNAs. We used miRNA microarrays to provide a detailed account of miRNA profiles for early passage and senescent human foreskin (BJ) fibroblasts as well as early and late passage immortalized fibroblasts (BJ-hTERT) that stably express the human telomerase reverse transcriptase subunit hTERT. The revelation that miRNA expression changes with extended passaging in BJ-hTERT cells will contribute to a comprehensive understanding of the connections between telomerase expression, senescence and processes of cellular aging.

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Transcriptome data from U2OS cells and hTERT CRISPR clones

Project description:Transcriptome data from U2OS cells and hTERT CRISPR clones

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Project description:Effect of CCAR2 depletion on the gene expression profile of BJ-hTERT cells

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Effect of CCAR2 depletion on the gene expression profile of BJ-hTERT and U2OS cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-03-08 | GSE87364 | GEO

hTERT promotes cell adhesion and migration independent of telomerase activity

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