ABSTRACT: Profiling of gut gene expression in Cry1Ab-resistant and susceptible strains of Ostrinia nubilalis reveals possible adaptations of resistant larvae to transgenic Cry1Ab corn
Project description:This microarray was used to monitor gene expression in early third-instar larvae of Bt-susceptible O. nubilalis after 6-h feeding on diet with or without Bacillus thuringiensis (Bt) Cry1Ab protoxin.We identified 174 transcripts, for which the expression was changed more than 2-fold in the gut of the larvae fed Cry1Ab protoxin (p<0.05), representing 80 down-regulated and 94 up-regulated transcripts. Among 174 differentially expressed transcripts, 13 transcripts putatively encode proteins that are potentially involved in Bt toxicity, and these transcripts include eight serine proteases, three aminopeptidases, one alkaline phosphatase, and one cadherin. Cry1Ab protoxin ingestion induced gene expression was measured in O. nubilalis gut at 6 after Cry1Ab protoxin toxin exposure to doses of 0.25 M-NM-<g/ml artificial diet. three independent experiments were performed using three insect gut samples,and each sample was from five insects' gut pooled.
Project description:This microarray was used to monitor gene expression in early third-instar larvae of Bt-susceptible O. nubilalis after 6-h feeding on diet with or without Bacillus thuringiensis (Bt) Cry1Ab protoxin.We identified 174 transcripts, for which the expression was changed more than 2-fold in the gut of the larvae fed Cry1Ab protoxin (p<0.05), representing 80 down-regulated and 94 up-regulated transcripts. Among 174 differentially expressed transcripts, 13 transcripts putatively encode proteins that are potentially involved in Bt toxicity, and these transcripts include eight serine proteases, three aminopeptidases, one alkaline phosphatase, and one cadherin.
Project description:This a reciprocal transplant experiment. Two hebivorous lepidoptera species (Ostrinia nubilalis and Ostrinia scapulalis) have been fed either their preferred plant (corn for O.nubilalis and Mugwort for O. scapulalis) or the reciprocal plant. At 4th larval instar, RNA was extracted from whole larvae, size selected to enrich in small RNAs and sequenced by Illumina single-end 50bp.
