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Escherichia coli strain:K-12 BW25113 HT2

ABSTRACT: Escherichia coli K-12 BW25113 HT2 genome sequencing

PROVIDER: PRJNA346574 | ENA |

REPOSITORIES: ENA

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Escherichia coli K-12: 5% isooctane treatment at four time points

Project description:Transcripitonal profiling of Escherichia coli K-12 BW25113 comparing cells with isooctane treatment at time point of 0, 10, 30 and 60 min with two biological replicates

2014-09-13 | GSE32131 | GEO

Escherichia coli strain:K-12 BW25113 HT4

Project description:Escherichia coli K-12 BW25113 HT4 genome sequencing

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E. coli BW25113 ompA mutant vs wild type biofilm and E. coli BW25113 ompA/pCA24N_ompA vs ompA/pCA24N suspension cells

Project description:This Series involves two studies: 1) The gene expression of E. coli K-12 BW25113 ompA mutant strain vs. wild type strain glasswool biofilm cells and E. coli K-12 BW25113 ompA mutant vs. wild type polystyrene biofilm cells. 2) The gene expression of E. coli BW25113 ompA/pCA24N_ompA vs. ompA/pCA24N suspension cells.

2010-01-20 | GSE14064 | GEO

E. coli BW25113 frdC vs. E. coli BW25113 HW2

Project description:Glycerol is an attractive feedstock for biofuels since it accumulates as a byproduct during biodiesel operations; hence, it is interesting to consider converting glycerol to hydrogen using the formate hydrogen lyase system of Escherichia coli which converts pyruvate to hydrogen. Starting with Escherichia coli BW25113 frdC that lacks fumarate reductase to eliminate the negative effect of accumulated hydrogen on glycerol fermentation and by using both adaptive evolution and chemical mutagenesis combined with a selection method based on increased growth on glycerol, we obtained an improved strain, HW2, that produces 20-fold more hydrogen in glycerol medium (0.68 mmol/L/h) compared to that of frdC mutant. HW2 also grows 5-fold faster (0.25 1/h) than BW25113 frdC on glycerol, so it achieves a reasonable growth rate. Corroborating the increase in hydrogen production, glycerol dehydrogenase activity in HW2 increased 4-fold compared to BW25113 frdC. In addition, a whole-transcriptome study revealed that several pathways that would decrease hydrogen yields were repressed in HW2 (fbp, focA, and gatYZ) while a beneficial pathway, eno which encodes enolase was induced.

2010-10-31 | GSE18972 | GEO

Escherichia coli transcriptome reorganization caused by nutritional changes

Project description:Escherichia coli K-12 BW25113 were cultured in the LB and M63 media. Exponetially growing populations were collected for RNAseq. The culture medium-induced transcriptional changes were analyzed.

2020-08-31 | GSE136101 | GEO

Expression Profiling of E. coli K-12 BW25113 in Minimal Media with Different Carbon Sources

Project description:mRNA sequencing was performed on E. coli K-12 BW25113 grown on Glucose, Pyruvate, Fumarate, and Acetate.

2017-02-14 | GSE59759 | GEO

Proteomic changes in Escherichia coli BW25113 ∆ompF and ∆waaF (LC-MSMS)

Project description:This data set was acquired as a control set for an evolutionary study enquiring the response of Escherichia coli on the protein level to predatory pressure by Bdellovibrio bacteriovorus. Predatory bacterium Bdellovibrio bacteriovorus is ubiquitous and has a broad bacterial prey range including many antimicrobial resistant pathogens. However, it is currently unclear whether prey bacteria can evolve genetically-determined resistance against predation by B. bacteriovorus. We found that the model bacterium Escherichia coli K-12 MG1655 evolves resistance against B. bacteriovorus HD100 during experimental evolution. The experimental evolution setting showed that E. coli K-12 MG1655 lineages evolved under high predatory pressure have reduced/or no level of outer membrane porin F (OmpF) next to other changes at the outer surface on the genome and proteome level. For the latter please refer to PRIDE submission PXD080500. As a control set the proteome was analysed of markerless single gene deletion mutants of ∆ompF and ∆waaF and their reference strain E. coli BW25113 (from the KEIO library). This data set reveals the impact a single deletion has in the E. coli genome on the proteome, which is useful to assess the interconnectedness of gene regulation, the wholistic influence on the proteins, and the effect on the E. coli surface. The latter is not only useful to understand predator-prey interaction more closely, but might contribute to a better understanding of E. coli physiology overall.

2026-09-20 | PXD080501 | Pride

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Project description:Escherichia coli BW25113 Genome sequencing

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