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Gremmeniella abietina

ABSTRACT: Gremmeniella abietina overview

PROVIDER: PRJNA347407 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6447990_subreads.fastq.gz	Fastqsanger.gz
	SRR6447982_subreads.fastq.gz	Fastqsanger.gz
	SRR6447983_subreads.fastq.gz	Fastqsanger.gz
	SRR6447984_subreads.fastq.gz	Fastqsanger.gz
	SRR6447985_subreads.fastq.gz	Fastqsanger.gz

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Description, Distribution, and Relevance of Viruses of the Forest Pathogen Gremmeniella abietina.

Project description:The European race of the ascomycetous species Gremmeniella abietina (Lagerberg) Morelet includes causal agents of shoot blight and stem canker of several conifers in Europe and North America, which are known to host a diverse virome. GaRV6 is the latest and sixth mycovirus species reported within G. abietina. Before its description, one victorivirus and one gammapartitivirus species were described in biotype A, two mitoviruses in both biotypes A and B and a betaendornavirus in biotype B. Possible phenotypic changes produced by mycoviruses on G. abietina mycelial growth have been reported in Spanish mitovirus-free and GaRV6-hosting G. abietina isolates, which had higher growth rates at the optimal temperature of 15 °C, but no other major differences have been observed between partitivirus-like dsRNA and dsRNA-free isolates. In this review, we reappraise the diversity of viruses found in G. abietina so far, and their relevance in clarifying the taxonomy of G. abietina. We also provide evidence for the presence of two new viruses belonging to the families Fusariviridae and Endornaviridae in Spanish isolates.

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Specific and sensitive detection of the conifer pathogen Gremmeniella abietina by nested PCR.

Project description:BackgroundGremmeniella abietina (Lagerb.) Morelet is an ascomycete fungus that causes stem canker and shoot dieback in many conifer species. The fungus is widespread and causes severe damage to forest plantations in Europe, North America and Asia. To facilitate early diagnosis and improve measures to control the spread of the disease, rapid, specific and sensitive detection methods for G. abietina in conifer hosts are needed.ResultsWe designed two pairs of specific primers for G. abietina based on the 18S rDNA sequence variation pattern. These primers were validated against a wide range of fungi and 14 potential conifer hosts. Based on these specific primers, two nested PCR systems were developed. The first system employed universal fungal primers to enrich the fungal DNA targets in the first round, followed by a second round selective amplification of the pathogen. The other system employed G. abietina-specific primers in both PCR steps. Both approaches can detect the presence of G. abietina in composite samples with high sensitivity, as little as 7.5 fg G. abietina DNA in the host genomic background.ConclusionThe methods described here are rapid and can be applied directly to a wide range of conifer species, without the need for fungal isolation and cultivation. Therefore, it represents a promising alternative to disease inspection in forest nurseries, plantations and quarantine control facilities.

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Russula abietina

Project description:Russula abietina Raw sequence reads

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Veluticeps abietina strain:OMC1657 | isolate:FBCC 2439

Project description:Veluticeps abietina OMC1657 Transcriptome - Ottogen9_T

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