Project description:During sub-zero (cryophilic) growth, nodular features appear on the cell surface of Planococcus halocryophilus; however, the biochemical composition of these features as well as any cold-adaptive benefits they may offer are not understood. This study aimed to identify differences in the surfaceome of P. halocryophilus cells grown under optimal (24°C) and sub-zero (-5°C and -10°C) culture conditions, for the purpose of gaining insight into cold-adapted proteomic traits at the cell surface.
Project description:This is the first whole-cell proteomic study performed on a subzero-growing permafrost-associated isolate at such a low temperature (-10C) and one that connects subzero growth and the salt response. Through whole-cell proteomic analyses at -10C, 23C, and high/low salt conditions, we could identify functional pathways and mechanisms important for subzero growth in the extremophile Planococcus halocryophilus.
