Project description:To explore the molecular mechanisms underlying selective differentiation of MAIT-derived iPSCs into reMAITs, we conducted micro RNAs profiling based on microarrays. We sampled reMAITs during the time course of differentiation from iPSCs as well as mature MAITs isolated from cord blood (CB MAITs). As control, we used immature T cells differentiated from hematopoietic stem cells (HSCs). For each of reMAITs and the immature T cells, we selected four time points: Start, Early, Middle, and Late. MAITs isolated from CB were reprogrammed to iPSCs (PMID:23523177). The MAIT-derived iPSCs were cultured on OP9, and CD34+ CD43+ cells were isolated. These CD34+ CD43+ precursor cells were furthered cultured on OP9/DL1 for re-differentiation into MAITs. Based on the observation of the reported surface antigen profiles (PMID:23523177), reMAITs were harvested at four different time points: day 0 (Start), day 4 (Early), day 7-10 (Middle), and after day 30 (Late). For the immature T cells, CD34+ cells were isolated from CB using CD34 MicroBead Kit (Miltenyi Biotech). These CD34+ HSCs were cultured on OP9/DL1 for differentiation into T cell lineage as previously described (PMID:15494433). Based on the surface antigen profiles, the immature T cells were harvested at four different time points: CD34+ cells at day 0 (Start), CD4- CD8- double negative cells at day 21 (Early) and day 40 (Midlle), and CD4+ CD8+ double positive cells after day 50 (Late). Total RNA was extracted from each sample using RNeasy Kit (Quiagen). For micro RNA analysis, RNA was labeled with Cy3-pCp by ligation, and subjected to analysis using Human miRNA Microarray Release 19.0 8x60K (Agilent).

2017-03-14 | GSE88936 | GEO

Homo sapiens

Project description:Post-infectious time-series DNA methylation analysis in gastric epithelial cells

| PRJNA351217 | ENA

Gene Expression Profile of 53 human T-ALL samples collected in human clinical trial E2993 (Marks et al., 2009)

Project description:To analyze the prognostic relevance of transcriptional profiling in adult T-ALL, we analyzed a clinical series of 53 primary leukemia samples uniformly treated according to the ECOG E2993 protocol using gene expression oligonucleotide microarrays. Unsupervised analysis and consensus clustering of microarray gene expression data in this series revealed the presence of 2 stable gene expression clusters corresponding to early immature (n = 28) and cortical/mature (n = 25) adult T-ALLs respectively. Early immature T-ALLs show a gene expression signature related to hematopoietic stem cells and myeloid progenitors that was recently linked to a group of childhood T-ALLs with poor prognosis. Notably, univariate analysis in our patient series confirmed that early immature adult T-ALL is associated with poor prognosis and reduced overall survival compared with cortical/mature adult T-ALL (P = 0.0197)

2013-08-02 | GSE42328 | GEO

Gene expression profiles of MAIT cells, MAIT-iPSCs, hiPSCs, hESCs, and reMAIT cells

Project description:To show the similarity among MAIT-iPSCs, hiPSCs and hESCs and the gradual change of global gene expression of reMAIT cells along with differentiation, this experiment was designed. MAIT cells, MAIT-iPSCs, hiPSCs, hESCs, MAIT cells, and reMAIT cells at the several differerent stages of differentiation were collected. Then, they were applied in this experiment.

2013-03-21 | E-GEOD-44559 | biostudies-arrayexpress

Homo sapiens

Project description:Time-series toxicogenomics analysis of N-nitroso compound exposure in Caco-2 cells

| PRJNA124507 | ENA

Homo sapiens

Project description:EMT Time series in ARPE19

| PRJNA112613 | ENA

Homo sapiens

Project description:Time series RNA profiling of endotheilal cells in response to atheroprone and atheroprotective flows

| PRJNA357824 | ENA