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Schizosaccharomyces pombe

ABSTRACT: Survival in quiescence requires the euchromatic deployment of Clr4/SUV39H by Argonaute-associated small RNAs [sRNA-Seq]

PROVIDER: PRJNA350403 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4449650.fastq.gz	Fastqsanger.gz
	SRR4449660.fastq.gz	Fastqsanger.gz
	SRR4449670.fastq.gz	Fastqsanger.gz
	SRR4449680.fastq.gz	Fastqsanger.gz
	SRR4449690.fastq.gz	Fastqsanger.gz

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Schizosaccharomyces pombe

Project description:Survival in quiescence requires the euchromatic deployment of Clr4/SUV39H by Argonaute-associated small RNAs [siRNA-Seq]

| PRJNA350506 | ENA

Schizosaccharomyces pombe

Project description:Survival in quiescence requires the euchromatic deployment of Clr4/SUV39H by Argonaute-associated small RNAs [ChIP-Seq]

| PRJNA350393 | ENA

Schizosaccharomyces pombe

Project description:Survival in quiescence requires the euchromatic deployment of Clr4/SUV39H by Argonaute-associated small RNAs [RNA-Seq]

| PRJNA350396 | ENA

Survival in quiescence requires the euchromatic deployment of Clr4/SUV39H by Argonaute-associated small RNAs

Project description:Survival in quiescence requires the euchromatic deployment of Clr4/SUV39H by Argonaute-associated small RNAs

| PRJNA350496 | ENA

Survival in quiescence requires the euchromatic deployment of Clr4/SUV39H by Argonaute-associated small RNAs [siRNA-Seq]

Project description:Cellular quiescence (G0) is a ubiquitous stress response through which cells enter a state of reversible dormancy, acquiring distinct properties including reduced metabolism, resistance to stress and long life. G0 entry involves dramatic changes to the chromatin landscape and transcriptional program of cells, but the mechanisms coordinating these processes remain poorly understood. Using the fission yeast, here we track the G0-associated chromatin and transcriptional changes temporally. We show that as cells enter G0, their survival and global gene expression programs become increasingly dependent on Clr4/SUV39H, the sole histone H3 lysine 9 (H3K9) methyltransferase, and RNA interference (RNAi) proteins. Notably, G0 entry results in RNAi-dependent H3K9 methylation of several euchromatic regions, prior to which Argonaute 1-associated small RNAs from these regions emerge. Overall our data reveal a new function for constitutive heterochromatin proteins in establishing the global G0 transcriptional program and suggest that stress-induced alterations in Argonaute-associated sRNAs can specify the deployment of transcriptional regulatory proteins to unique sites in eukaryotic genomes.

2016-12-05 | GSE89150 | GEO

Survival in quiescence requires the euchromatic deployment of Clr4/SUV39H by Argonaute-associated small RNAs [sRNA-Seq]

2016-12-05 | GSE89149 | GEO

Survival in quiescence requires the euchromatic deployment of Clr4/SUV39H by Argonaute-associated small RNAs [RNA-Seq]

2016-12-05 | GSE89145 | GEO

Survival in quiescence requires the euchromatic deployment of Clr4/SUV39H by Argonaute-associated small RNAs [ChIP-Seq]

2016-12-05 | GSE89142 | GEO

Schizosaccharomyces pombe

Project description:Automethylation-induced conformational switch in Clr4 (Suv39h) maintains epigenetic stability

| PRJNA399201 | ENA

RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly

Project description:In eukaryotes, the Suv39 family of proteins tri-methylate lysine 9 of histone H3 (H3K9me) to form constitutive heterochromatin. However, how Suv39 proteins are nucleated at heterochromatin is not fully described. In the fission yeast, current models posit that Argonaute1-associated small RNAs (sRNAs) nucleate the sole H3K9 methyltransferase, Clr4/SUV39H, to centromeres. Here, we show that in the absence of all sRNAs and H3K9me, the Mtl1 and Red1 core (MTREC)/PAXT complex nucleates Clr4/SUV39H at a heterochromatic long noncoding RNA (lncRNA) at which the two H3K9 deacetylases, Sir2 and Clr3, also accumulate by distinct mechanisms. Iterative cycles of H3K9 deacetylation and methylation spread Clr4/SUV39H from the nucleation center in an sRNA-independent manner, generating a basal H3K9me state. This is acted upon by the RNAi machinery to augment and amplify the Clr4/H3K9me signal at centromeres to establish heterochromatin. Overall, our data reveal that lncRNAs and RNA quality control factors can nucleate heterochromatin and function as epigenetic silencers in eukaryotes.

2024-05-16 | PXD039519 | Pride

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