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Streptococcus pneumoniae

ABSTRACT: IPTG-induced CRISPRi repression of firefly luciferase in Streptococcus pneumoniae

PROVIDER: PRJNA353113 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5010973.fastq.gz	Fastqsanger.gz
	SRR5010974.fastq.gz	Fastqsanger.gz
	SRR5010975.fastq.gz	Fastqsanger.gz
	SRR5010976.fastq.gz	Fastqsanger.gz
	SRR5010977.fastq.gz	Fastqsanger.gz

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IPTG-induced CRISPRi repression of firefly luciferase in Streptococcus pneumoniae

Project description:Genome-wide screens have discovered a large set of essential genes in the human pathogen Streptococcus pneumoniae. However, the function of many essential genes is still unknown, hampering vaccine and drug development programs. Based on results from transposon-sequencing (Tn-Seq), we refined the list of essential genes in S. pneumoniae serotype 2 strain D39. Next, we created a knockdown library targeting all 391 potentially essential genes using CRISPR interference (CRISPRi). Using high-content microscopy screening, we searched for essential genes of unknown function with clear phenotypes in cell morphology upon CRISPRi-based depletion. We identified SPD1416 and SPD1417 (named to MurT and GatD, respectively) as essential peptidoglycan synthesis proteins and we show that SPD1198 and SPD1197 (named to TarP and TarQ, respectively) are responsible for the polymerization of teichoic acid (TA) precursors. This knowledge enabled us to reconstruct the unique pneumococcal TA biosynthetic pathway. Our CRISPRi library provides a valuable tool for characterization of pneumococcal genes and pathways and revealed several promising antibiotic targets. This RNA-Seq dataset is aimed to show that induction of the CRISPRi system very selectively represses its target gene, firefly luciferase, without other observable transcriptional effects.

2017-03-03 | GSE89763 | GEO

Identification of Proteins in Streptococcus pneumoniae extracellular vesicles (pEVs)

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CcpA Ensures Optimal Metabolic Fitness of Streptococcus pneumoniae

Project description:This SuperSeries is composed of the following subset Series: GSE31815: ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + Glucose at MID-log growth phase GSE31816: ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + GLucose at transition-phase of growth (TS) GSE31817: ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + Galactose at MID-log growth phase GSE31818: ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + galactose at transition-phase of growth (TS) Refer to individual Series

2011-09-30 | E-GEOD-31819 | biostudies-arrayexpress

Transcriptional response of Streptococcus pneumoniae to copper

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in CDM with 0mM to 0.05 mM copper.

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Transcriptional response of Streptococcus pneumoniae D39 to Zinc

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in CDM Plus 0mM Zn2+ to grown in CDM plus 0.2 mM Zn2+.

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Bioplatforms Australia: Antibiotic Resistant Sepsis Pathogens Framework Initiative - Streptococcus pneumoniae 4559

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Bioplatforms Australia: Antibiotic Resistant Sepsis Pathogens Framework Initiative - Streptococcus pneumoniae 947

2021-07-01 | GSE152965 | GEO

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