Project description:Plants acclimate to environmental fluctuations by transitory reconfigurations the homeostatic network. Primary studies suggested that transcriptome responses to deal with fluctuations in light intensity and temperature tend to reversibility after stress removal in the model plant Arabidopsis thaliana. To gain more insight into this pattern in the context of acclimation, RNA-Seq analysis were conducted in Arabidopsis thaliana after different abiotic stress treatments consisting in high light (HL), high humidity, drought, heat, cold and combinations among factors or after recovery periods. Our transcriptome study is in line of a general pattern wherby transcriptome changes in response to adverse environments are prone to return to the basal state during the de-acclimation phase.
Project description:Secondary metabolites are involved in the plant stress response. Among these are scopolin and its active form scopoletin, which are coumarin derivatives associated with reactive oxygen species scavenging and pathogen defence. Here we show that in Arabidopsis thaliana, scopolin accumulation can be induced in the root by osmotic stress and in the leaf by low temperature stress. A genetic screen for altered scopolin levels in Arabidopsis thaliana identified a mutant compromised for scopolin accumulation in response to stress; the lesion was present in a homologue of THO1, the product of which contributes to the THO/TREX complex. The THO/TREX complex contributes to RNA silencing, supposedly by trafficking precursors of small RNAs. Mutants carrying defective THO and RDR6 genes were impaired with respect to scopolin accumulation in response to stress, suggesting a mechanism based on RNA silencing like the transacting small interfering RNA pathway which requires THO/TREX and RDR6 function.
Project description:High-throughput sequencing of Arabidopsis thaliana endogenous small RNAs by 454 pyrosequencing. Keywords: high-throughput sequencing
Project description:Expression profiles of MicroRNA and SiRNA of Arabidopsis thaliana Col-0 and transgenic plants with constitutive expression of the chimeric receptors NRG1 grown at different temperature To reveal the underlying molecular mechanism of de-cosuppression with memory by high temperature in Arabidopsis, we performed the expression profiles of microRNA and SiRNA in transgenic plants with constitutive expression of the chimeric receptors NRG1 and wide type Col-0 grown at different temperature using the Custom LC Sciences Arabidopsis microRNA and SiRNA array. Keywords: high temperature, de-cosuppression, MicroRNA, SiRNA
Project description:To investigate the impact of a plant´s response to abiotic stress on plant defense against subsequent biotic stress, we determined the transcriptional response of Arabidopsis thaliana to low temperature stress (4°C) and subsequent mechanical wounding or larval feeding damage by the herbivores Mamestra brassicae (generalist) and Pieris brassicae (specialist). In total, 21%, 4% and 14% of all genes responsive to M. brassicae, P. brassicae or mechanical wounding were differentially regulated in previously cold-treated compared to untreated plants.
