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Clostridioides difficile

ABSTRACT: Clostridioides difficile Genome sequencing

PROVIDER: PRJNA353581 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5044091_1.fastq.gz	Fastqsanger.gz
	SRR5044091_2.fastq.gz	Fastqsanger.gz
	SRR5044092_1.fastq.gz	Fastqsanger.gz
	SRR5044092_2.fastq.gz	Fastqsanger.gz
	SRR5044093_1.fastq.gz	Fastqsanger.gz

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Clostridioides difficile responses to calprotectin

Project description:The intestines house a diverse microbiota that must compete for nutrients to survive, but the specific limiting nutrients that control pathogen colonization are not clearly defined. Clostridioides difficile colonization typically requires prior disruption of the microbiota, suggesting that outcompeting commensals for resources is key in establishing C. difficile infection (CDI). The immune protein calprotectin (CP) is released into the gut lumen during CDI to chelate zinc (Zn) and other essential nutrient metals. Yet, the impact of Zn limitation on C. difficile colonization is unknown. To define C. difficile responses to Zn limitation, we performed RNA sequencing on C. difficile exposed to CP. In media with CP, C. difficile upregulated genes involved in metal homeostasis and amino acid metabolism.

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Clostridioides difficile toxins deregulate tight junction proteins.

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Clostridioides difficile

Project description:Clostridioides difficile Genome sequencing

| PRJNA202879 | ENA