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Mus musculus

ABSTRACT: A label-retaining but unipotent cell population resides in biliary compartment of mammalian liver.

PROVIDER: PRJNA354605 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5044970.fastq.gz	Fastqsanger.gz
	SRR5044971.fastq.gz	Fastqsanger.gz
	SRR5044972.fastq.gz	Fastqsanger.gz
	SRR5044973.fastq.gz	Fastqsanger.gz
	SRR5044974.fastq.gz	Fastqsanger.gz

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A label-retaining but unipotent cell population resides in biliary compartment of mammalian liver.

Project description:Cells with slow proliferation kinetics that retain the nuclear label over long time periods – the label-retaining cells (LRCs) – represent multipotent stem cells in a number of adult tissues. Since the identity of liver LRCs (LLRCs) had remained elusive we utilized a genetic approach to reveal LLRCs in normal non-injured livers and characterized their regenerative properties in vivo and in culture. We found that LLRCs were located in biliary vessels and participated in the regeneration of biliary but not hepatocyte injury. In culture experiments the sorted LLRCs displayed an enhanced self-renewal capacity but a unipotent biliary differentiation potential. Transcriptome analysis revealed a unique set of tumorigenesis- and nervous system-related genes upregulated in LLRCs when compared to non-LRC cholangiocytes. We conclude that the LLRCs established during the normal morphogenesis of the liver do not represent a multipotent primitive somatic stem cell population but act as unipotent biliary progenitor cells.

2017-03-01 | GSE90160 | GEO

Mus musculus

Project description:Expression profile of sweat gland label retaining cells (LRCs)

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Mus musculus

Project description:Intestinal label-retaining cells are secretory precursors expressing Lgr5

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Murine Kidney Papilla Cells: Control GFP- vs. GFP+ label retaining cells. Cell populations were separated by FACS.

Project description:Transcriptional profiling of mouse kidney papilla cells comparing control GFP- cells with GFP+ labeled cells. Mouse kidney papillae were dissected. GFP- and GFP+ cell populations were isolated by FACS. The latter represent slow cycling label retaining cells (LRCs). Two-condition experiment, GFP- vs. GFP+ label retaining cells cells. Biological replicates: 5 control GFP- replicates, 5 GFP+ label retaining cell replicates.

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Benary2021 - tissue dynamics of the hepato-pancreato-biliary organ system

Project description:Single cell-based studies have revealed tremendous cellular heterogeneity in stem cell and progenitor compartments, suggesting continuous differentiation trajectories with intermixing of cells at various states of lineage commitment and notable degree of plasticity during organogenesis. The hepato-pancreato-biliary organ system relies on a small endoderm progenitor compartment that gives rise to a variety of different adult tissues, including liver, pancreas, gallbladder, and extra-hepatic bile ducts. Experimental manipulation of various developmental signals in the mouse embryo underscored important cellular plasticity in this embryonic territory. This is also reflected in the existence of human genetic syndromes as well as congenital or environmentally-caused human malformations featuring multiorgan phenotypes in liver, pancreas and gallbladder. Nevertheless, the precise lineage hierarchy and succession of events leading to the segregation of an endoderm progenitor compartment into hepatic, biliary, and pancreatic structures are not yet established. Here, we combine computational modelling approaches with genetic lineage tracing to assess the tissue dynamics accompanying the ontogeny of the hepato-pancreato-biliary organ system. We show that a multipotent progenitor domain persists at the border between liver and pancreas, even after pancreatic fate is specified, contributing to the formation of several organ derivatives, including the liver. Moreover, using single-cell RNA sequencing we define a specialized niche that possibly supports such extended cell fate plasticity.

2021-07-01 | MODEL2105030001 | BioModels

ATF4 marks quiescent reserve cells of gastric chief cells

Project description:Hierarchically organized tissues, such as hematopoietic systems, muscle, or skin harbor deeply quiescent stem cells which start proliferating in response to external insults. In contrast, it remains obscure whether similar quiescent cells exist in epithelia of digestive organs. Here we identified a deeply quiescent population in gastric corpus but not in other gastrointestinal organs after systematic examination of H2b-GFP label-retaining cells. The label-retaining cells in corpus epithelia belonged to a subpopulation of chief cells that were located near basal layers of corpus and did not overlap with Troyhigh, Lgr5high, or Misthigh cell population. The identified quiescent cells were marked with activation of Atf4 and unfolded protein response. External damages by indomethacin treatment triggered proliferation of the quiescent populations, indicating that chief cells of gastric corpus harbor deeply quiescent reserve cells with high levels of internal stress response activity.

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Aging induces aberrant state transition kinetics in murine muscle stem cells

Project description:Label retaining and non-retaining muscle stem cells from young and aged H2B-GFP+/-;rtTA+/- were profiled by single cell RNA-seq at two timepoints

2020-02-18 | GSE143476 | GEO

HMEC Label-retaining cells

Project description:Gene expression after mammosphere culture, label-retaining cells

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HMEC Non-label-retaining cells

Project description:Gene expression after mammosphere culture, non-label-retaining cells

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2021-09-09 | PXD020974 | Pride

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